The leaves of the Capparisspinosa were collected from the eastern Radwaniya/Baghdad duringthe months April, May, June, July, September, and September. The leaves were extracted by water and alcohol. The effectiveness of anti-tumor and anti-leishmania for the water and alcohol extracts of al-Qabar leaf samples for May and September, which were characterized by the highest percentage of active compounds for the rest of the tested months, was evaluated by adopting the results of a previous study for the same researchers or chemical composition and leaf content of active compounds The concentrations of 250, 500, 1000 mg.L 1 were evaluated for anti-tumor efficacy. The anti-leishmania efficacy was tested at 250, 500 and 1000 mg L 1. The sample of the alcohol extract for May collected complex showed the highest anti-tumor activity at 63.5% at 500 mg.L 1 and the highest efficacy of the samples of alcohol extract for September was 56.1% at the concentration of 250 mg.L 1 , while the water extract for the May sample did not show any effectiveness even at the highest concentration 1000 mg L 1 , but the September collected sample was effective at 49.3% In the case of the inhibitory efficacy of leishmania, the alcohol extract of the May sample did not show on inhibitory activity at the lowest concentration. The effectiveness of 41.3% was observed at 500 mg.L 1 , but decreased to 14.8% at the highest concentration of 1000 mg L-1. Of the same month at the lowest concentration of 37.8% and decreased effectiveness there was an increase in the anti-leishmania effect of the September extract of the sample with increased concentration. There was of its effectiveness at the lowest concentration of a user and then reached 8 and 37% at the concentration of 500 and 1000 mg.L 1 with the same percentage, and the water extract for the September sample did not show any efficacy at the minimum and highest concentrations, except the concentration of 500 mg.L 1 , which reached 11%.
Back ground: Rice bran is an industrial waste resulting from the grain milling process. Consisting of husk, aleurone, and fractions. It contains many nutrients, including soluble and insoluble fiber, vitamins, minerals, fats, proteins, and phytochemicals such as Ɣ-oryzanol, tocopherols, tocotrienols and tricin which has health-beneficial properties. Objective: The aim of research to estimate antioxidant activity of the aqueous extract of rice bran of the commercial jasmine variety and the tricin compound purified from it, by the method of nitric oxide suppression activity and the effectiveness of inhibiting lipid peroxidation, and the effect of the temperatures (63, 72,72, 85,100, 121,121, and 138) Co and the pH (4, 5, 6, 7, 8) in the activity. Materials and methods: Samples of rice bran of the commercial jasmine variety were collected from Al-Tahbeesh sites in Al-Najaf Al-Ashraf for the year (2021). The aqueous extract was prepared using the maceration in boiling distilled water with a temperature of 70 C°, and the crystals of tricin were isolated by using a silica gel column (60) and solvents recovery n-hexane, ethyl acetate and methanol, respectively. This process resulted in nine parts (A-I) of the ethyl acetate solvent, concentrated in a rotary evaporator and left in the refrigerator 48 hours with the addition of chloroform to it. Needle-shaped yellow crystals were obtained, which were used to estimate antioxidant activity. Results: The effectiveness of purified tricin as an ability to inhibit nitric oxide was higher than that of the aqueous extract of rice bran and ascorbic acid at percentages of (97.82, 72.99 and 77.20)% respectively at a concentration of 400 µg / ml, the highest concentration used, also tricin was superior in inhibiting lipid peroxidation of 98.31% over the aqueous extract of rice bran of the commercial jasmine variety 81.28% and ascorbic acid 93.10 at the same concentration, purified tricin showed stability towards temperatures at times (30 min, 15 sec, 30 min, 1 min, 30 min, 5 min, 15 min and 2 sec) higher than that of the aqueous extract in both methods, as the effectiveness ratios of tricin by nitric oxide suppression method reached (64.25, 53.87, 42.78, 47.44, 38.48, 45.74 , 39.04 and 36.13)% respectively, and by the lipid peroxide suppression method (90.73, 85.06, 63.32, 77.50, 32.27, 76.65, 35.06 and 25.30) % respectively, the two methods of effectiveness were used to estimate the stability of the aqueous extract of rice bran and the tricin towards different pH. The effectiveness of tricin was higher than the aqueous extract of rice bran at all tested numbers, and the highest effectiveness of tricin at pH (pH7) with the method of inhibiting nitric oxide and inhibiting lipid peroxidation was (46.56 and 90.92)% respectively. Conclusion: purified tricin higher antioxidant activity than the aqueous extract using both methods, and showed higher stability towards changes in temperature and during different time periods, and higher stability towards a change in pH, especially in neutral conditions compared to acidic conditions and basal.
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