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Background: Infection is a possible problem in operating rooms. The aseptic technique is one of the circulating nurses’ duties to prevent infections and keep patients safe against microorganisms in the operating room. Objectives: This study aimed to assess the circulating nurses’ aseptic practices in orthopedic and general surgeries. Methods: This cross-sectional study was conducted on 296 circulating nurses who worked in public hospitals in Shiraz, Iran, during the 2020 summer. Data collection tools included a demographics questionnaire and the Aseptic Practices among Circulating Nurses Scale. The data were collected after the assessment of the psychometric properties of the tool. The data were analyzed using SPSS software (version 22). Results: The mean score of the aseptic practice scale was 3.50 out of 5. Although the aseptic practice score was higher than the average level, the circulating nurses had low scores in several items. Age and work experience had a weak negative correlation with the “establishment of a sterile field” subscale. The aseptic practice score was significantly different in circulating nurses with different academic educational levels. Conclusions: There is a need to improve some aseptic practices. Supplying the hospitals with necessary materials, using appropriate guidelines and educational programs, supervising, and setting appropriate policies can help improve aseptic practices.

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