The Gram-positive pathogen possesses type IV pili (TFP), which are extracellular fibers that are polymerized from a pool of pilin monomers in the cytoplasmic membrane. Two proteins that are essential for pilus functions are an assembly ATPase (PilB) and an inner membrane core protein (PilC). Two homologues each of PilB and PilC are present in, called PilB1/PilB2 and PilC1/PilC2, respectively, along with four pilin proteins, PilA1 to PilA4. The gene encoding PilA2, which is considered the major pilin based on previous studies, is immediately downstream of the and genes. Purified PilB2 had ATPase activity, bound zinc, formed hexamers even in the absence of ATP, and bound the second messenger molecule cyclic di-GMP (c-di-GMP). Circular dichroism spectroscopy of purified PilC2 indicated that it retained its predicted degree of alpha-helical secondary structure. Even though no direct interactions between PilB2 and PilC2 could be detected or even in the presence of c-di-GMP, high levels of expression of a diguanylate cyclase from (CPE1788) stimulated polymerization of PilA2 in a PilB2- and PilC2-dependent manner. These results suggest that PilB2 activity is controlled by c-di-GMP levels but that PilB2-PilC2 interactions are either transitory or of low affinity, in contrast to results reported previously from studies of the PilB1/PilC1 pair in which PilC1 was needed for polar localization of PilB1. This is the first biochemical characterization of a c-di-GMP-dependent assembly ATPase from a Gram-positive bacterium. Type IV pili (TFP) are protein fibers involved in important bacterial functions, including motility, adherence to surfaces and host cells, and natural transformation. All clostridia whose genomes have been sequenced show evidence of the presence of TFP. The genetically tractable species was used to study proteins involved in polymerizing the pilin, PilA2, into a pilus. The assembly ATPase PilB2 and its cognate membrane protein partner, PilC2, were purified. PilB2 bound the intracellular signal molecule c-di-GMP. Increased levels of intracellular c-di-GMP led to increased polymerization of PilA2, indicating that Gram-positive bacteria use this molecule to regulate pilus synthesis. These findings provide valuable information for understanding how pathogenic clostridia regulate TFP to cause human diseases.

The knowledge literature suggests that transferring knowledge leads to synergistic cost advantages, better implementation of organizational strategies, and competitive advantage. Organizations are implementing corporate universities to aid in knowledge transfer. There is no standardized definition for corporate universities, but rather models that allow organizations to customize them to meet their training needs. Building on recent work of managing the knowledge transfer process (Murray & Peyrefitte, 2007) and on seminal work on media richness theory (Daft & Lengel, 1986), the authors propose that the type of knowledge to be transferred, and the appropriate media to transfer that knowledge, determine the most beneficial generation of corporate university to achieve competitive advantage. The article presents a model and propositions concerning relationships between the type of knowledge to be transferred, appropriate media selection, and generation of corporate university to implement.

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The knowledge literature suggests that transferring knowledge leads to synergistic cost advantages, better implementation of organizational strategies, and competitive advantage. Organizations are implementing corporate universities to aid in knowledge transfer. There is no standardized definition for corporate universities, but rather models that allow organizations to customize them to meet their training needs. Building on recent work of managing the knowledge transfer process (Murray & Peyrefitte, 2007) and on seminal work on media richness theory (Daft & Lengel, 1986), the authors propose that the type of knowledge to be transferred, and the appropriate media to transfer that knowledge, determine the most beneficial generation of corporate university to achieve competitive advantage. The chapter presents a model and propositions concerning relationships between the type of knowledge to be transferred, appropriate media selection, and generation of corporate university to implement.

Trust in the work environment often has been associated with being a positive antecedent to many desirable organizational outcomes. Developing favorable organizational relationships builds trust, which might lead to an increase in job satisfaction and a positive attitude toward honoring psychological contracts. Using a quantitative research design, the present study explored the relationship between organizational trust and the related variables of job satisfaction and psychological contracts. An online survey was administered across various regions of the United States and information from 144 participants was collected. A Pearson's correlation technique was utilized to analyze the data collected and two significant findings emerged. First, there is a strong positive correlation between organizational trust and psychological contracts (employer obligations). This finding indicates employees who perceive their employer has fulfilled organizational obligations and commitment and honored all psychological contracts, both perceived and implied, have greater perceptions of organizational trust. The second finding showed a positive, moderate relationship exists between organizational trust and job satisfaction. Therefore, increases in organizational trust are associated with increases in job satisfaction and vice versa.