This study evaluated the effect of contamination by crude oil at different concentrations (0, 0.5, 1, 2, 4 and 8% w/w) in 3 lines of the corn plant (Zea mays) Creole, hybrids H384A and H386A. Variables such as germination, height, stem diameter, and leaf length-width were evaluated at 7, 10, 14 days after sowing (DDS). For each concentration of crude oil, three repetitions were made to obtain 54 experimental units, 18 for each of the lines studied (Criollo, H-384A, H-386A).In the present work we found that the Creole corn and the hybrid H386A, reached 100% germination at 5 DDS, while the hybrid H384A at 4 DDS at the different concentrations. Plant height, stem diameter and leaf size were affected in their development at higher concentrations of crude oil. The hybrid H384A showed a better response compared to the native corn and the hybrid H386A against contamination by crude oil. However, the native corn showed a favorable response to the different hydrocarbon concentrations. It is necessary to carry out more studies related to native maize races, to know the response to environmental contingencies such as oil spills.