Leaves of Gymnema sylvestre were extracted by hot and cold maceration.  Qualitative phytochemical analysis of the plant extract showed the presence of majority of compounds like alkaloids, carbohydrates, flavonoids, terpienoids, glycosides, proteins, amino acids, phenolic compounds and tannins..  Among the two extracts, the            hydro- alcoholic extract prepared by the hot maceration has shown high phenol content 6.51 ± 0.231 mg/g, high total favonolid content 89.51 ± 0.100 mg/g. By DPPH method, total antioxidant capacity, reducing power ability of the               hydro-alcoholic extract prepared by hot maceration process was evaluated. The freshly prepared rat hepatocytes were treated with different concentration of hydro-alcoholic extract prepared by the hot maceration process. The antihepatotoxicity produced by the extract at the concentration of 200, 400,600 µg/ml was found effective against the          D – galactosamine-induced hepatotoxicity, where as at the concentration of 800 µg/ml was found to be cytotoxic. A significant increase in the levels of ASAT, ALAT,ALP, total bilirubin, direct bilirubin ( P< 0.001) were observed. The cells were treated with  the hydro-alcoholic extract different extracts of Gymnema sylvestre showed a significant restoration of the altered biochemical parameters towards the normal (P<0.001) when compared to D- galactosamine treated groups and were found to be  dose  dependent.  A similar result was obtained when D- galactosamine intoxicated hepatocytes were treated with silymarin.

Azo dyes and their degradation products frequently show potential carcinogenic and genotoxic effects and can cause potential hazards to consumer health.