In this study, we report the first detection of P. aeruginosa ST 1076 harboring the bla gene in African countries in two cities (Annaba and Skikda) in northeastern Algeria. Additionally, we report the first detection of bla in K. pneumoniae ST 404 and ST 219 in Algerian cities (Annaba and Skikda).

Acquired metallo-β-lactamases (MβL) are emerging determinants of resistance in Pseudomonas aeruginosa. The objectives of this study were to phenotypically detect MβL in P. aeruginosa collected in urology ward from Skikda hospital Algeria. A total of seventeen P. aeruginosa isolates were identified using API 20NE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALD-TOFMS). Antibiotic susceptibility was performed using disk diffusion method on Muller-Hinton agar. The minimum inhibitory concentrations (MIC) of imipenem were determined by Etest method. Positively screened isolates were further subjected to four different methods phenotypic; Modified Hodge test (MHT), Imipenem-EDTA combined disk test (CDT), Imipenem-EDTA double-disk synergy test (DDST) and new biochemical method Modified Carba NP test (MCNP). Out of 32 (45.71%) isolates were resistant to imipenem; 20 (62,5%) isolates were MβL producing, 5 (15.62%) were carbapenemase class A or D producing, and 7 (21.87%) isolates were detected as negative test. Rapid detection of MβLproducing P. aeruginosa may help inappropriate antimicrobial therapy and avoid the development and dissemination of these strains. Thus far, the validation of a simple and accurate MβL detection method such as CDT, DDST and MCNP test, can be easily incorporated into the daily routine of a clinical laboratory.