Background: Health care-associated infection (HCAI) is a major problem for patient safety. Personal protective equipment (PPE) as defined by OSHA is specialized clothing or equipment, worn by an employee for protection against infectious materials. So, Proper education of health care workers (HCWs)about PPE is very important in clinical teaching and consider essential part of the undergraduate nursing programs. Web-based learning is rapidly emerging as a predominant paradigm in the delivery of education in society and it can be efficiently used for different types of education. It is platform independent, convenient in access, cost saving, easily updated contents and with emerging technologies it can be made more effective. The aim of this study is to assess the effect of web-based learning on academic achievement of community health nursing students in personal protective equipment module. Method: A randomized control trial research was used. The study was carried out in skill lab of community health nursing at faculty of nursing, Mansoura University from February to May 2017. The subjects of the study included 140 community health nursing students. These students were assigned randomly into two groups: web based group which included 70 students studying with web based learning module (WBL) and traditional group which included 70 students studying with traditional learning using face to face lecture and demonstration. Four tools were used for data collection: Structured self-administered questionnaire to assess students' socio demographic &occupational characteristics, students' knowledge about PPE and students' attitude toward web based learning and finally the practice assessment checklist. Results: the students in the web based group had significantly higher scores on both personal protective equipment knowledge and skill learning than students in the traditional group (P≤ 0.01).The students also showed positive attitude toward the web-based learning module. Conclusion: The study concluded that both traditional and web-based learning resulted in improvement of knowledge and practice regarding PPE. However, the web-based learning was more effective than traditional learning in increasing students' knowledge and improving skills regarding PPE.