This study presents experimental and numerical investigation for three-dimensional heat transfer characteristics in a turbine blade. An experimental setup was installed with a turbine cascade of five-blade channels. Blade heat transfer measurements were performed for the middle channel under uniform heat flux boundary conditions. Heat was supplied to the blades using twentynine electric heating strips cemented vertically on the outer surface of the blades. Distributions of heat transfer coefficient were obtained at three levels through blade height by measuring surface temperature distribution using thermocouples. To understand heat transfer characteristics, surface static pressure distributions on blade surface were also measured. Numerical investigation was performed as well to extend the investigation to locations other than those measured experimentally. Three-dimensional nonisothermal, turbulent flow was obtained by solving Reynolds averaged Navier-Stokes equations and energy equation. The shear stress transport -model was employed to represent turbulent flow. It was found through this study that secondary flow generated by flow deflection increases heat transfer coefficient on the blade suction surface. Separation lines with high heat transfer coefficients were predicted numerically with good agreement with the experimental measurements.