Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty-four compounds. GC/MS chromatogram analysis suggested the presence of opioid α-N-Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti-fungal, and anti-oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC 50 = 78.38 � 0.15) which suggests its antioxidant potential.
Aim and Objectives: The aim of this study was to assess and compare the changes in haematological parameters in neonates using cord blood from preeclamptic and from normal pregnancies. Materials and Methods: This is a cross sectional study based on Umbilical Cord Blood (UCB) samples taken from 40 preeclamptic and 40 normal pregnancies. 2 ml cord blood was collected immediately after delivery and transferred into an EDTA vial for CBC analysis and reticulocyte count was done using brilliant cresyl blue. The data entry and analysis was done on Microsoft Office Excel 2007. In all statistical analysis a p value<0.05 was considered significant. Results: RBC, Reticulocyte count, Reticulocyte Production Index (RPI) were found to be higher in neonatal cord blood from preeclamptic females than those from normal pregnancies. Haemoglobin and Haematocrit were found to be lower in neonatal cord blood from preeclamptic pregnancies. Conclusion: Newborns of preeclamptic mothers should be carefully monitored in terms of hematologic abnormalities. Thus, suspecting a deranged haematological profile early in the course of disease will be of great help in the preemptive management strategies that have proven to be playing a crucial role time and again in reducing the morbidities and mortalities associated with Preeclampsia.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.