Burnout is considered to be the challenges that have made the life of workers unbearable especially with respect to healthcare professionals. Therefore, this research is aimed at examining factors influencing burnout among nurses working in Obafemi Awolowo University Teaching Hospitals complex, Osun State, Nigeria. The research adopted a quantitative descriptive survey research design. The sample size of 280 was determined by Taro’s formular. A self-structured questionnaire was validated and was used to collect data. Data collected were analyzed using descriptive and inferential statistics at 0.05 level of significance. The result shows that most of the respondents (84.4%) were affected by the burnout because majority of them agreed that the burnout can occur as a result of increased work load with a mean score of 4.80. Majority also claimed that poor remuneration could contribute to burnout since they are likely to have little or nothing to show for their consistent exhaustion, these respondents have a mean value of 4.30. Overall, the mean score of burnout of the respondents was 4.44. This implies that most of the respondents were moderately affected by the burnout among nurses. Further results on the hypotheses shows that there was a positive and low correlation between work-related factors and environmental factors influencing burn out among nurses (r=.377, p<0.0.01). Also, there was a positive and low correlation between work related factors and personal factors influencing burn out among nurses (r=.329, p<0.0.01). The research concluded work-related, personal and environmental factors were established as strong factors contributing to the effect of burnout among nurses. It is recommended that government agencies and medical practitioners should device ways by which burnout can be prevented among employees.