The kinetics of thermal inactivation of Bacillus stearothermophilus spores in water extracts of soybeans (“soymilk”) was studied using a pilot scale scraped-surface heat exchanger. Survivor curves followed typical first-order inactivation reactions, with D-values ranging from 21.9 sec at 259 F to 5.3 sec at 268 F. The z-value was 15 F, corresponding to an activation energy of 88.6 kcal/mole. Lethal effects in the heating and cooling cylinders accounted for more than 50% of the inactivation above a heater outlet (holding) temperature of 265 F.

Four cases are reported in which the patients' sera agglutinated all erythrocytes tested, including their own, provided the cells first had been freshly washed with saline solution. The reactions of these sera are described and compared with other reports of panagglutination and autoagglutination specifically related to the use of common laboratory solutions (albumin, saline, citrate, or oxalate).

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