This paper discusses the successful implementation of non-methanol carbon sources at the Littleton Englewood Wastewater Treatment Facility utilizing TETRA® deep-bed denitrification filter and a second facility utilizing a Parkson DynaSand® upflow deep bed continuous backwash filter. This paper will describe the methodology employed to implement nonmethanol carbon sources, the modifications required to utilize non-methanol carbon sources in deep bed filter technologies, lessons learned and full scale data from each project.

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The scope of this research is to determine the fate of glycerin COD using intensive monitoring equipment and sampling program at full scale facilities in conjunction with phosphorus uptake rate tests to determine the relative fraction of PAOs with denitrifying activity.

In wastewater treatment plants, the performance of the biological denitrification processes depend on the key functionally relevant microbial populations, which are affected by many factors, including influent characteristics (e.g., carbon sources), operational conditions, and temperature. This study used Automated Ribosomal Intergenic Spacer Analysis (ARISA) to analyze microbial population structures and dynamics associated with various carbon sourcespecific denitrifying enrichment cultures and reveal the dynamics and microbial diversity, as well as biochemical fundamentals underlying the different denitrification behavior observed. The ability of the specific carbon-acclimated populations to use other alternative carbon sources and the associated changes in microbial community structure was assessed by evaluating the maximum denitrification rates and with ARISA analysis. Although the ARISA profiles showed distinct community structures for denitrifying cultures as a result of enrichment with different carbon sources, no stable community structure or strong correlation between the microbial community signatures with certain carbon sources emerged. Furthermore, the ability of carbonspecific acclimated sludge to utilize other carbon sources varied for different enrichments, showing different acclimatization time duration and denitrification rates .

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