A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of cDNA pools prepared by global amplification PCR allowed discrim-inating choices to be made in selection of expressed sequence tags (ESTs) to be isolated. Sequencing of selected ESTs confirmed that both known and novel genes can be isolated from any developmental stage of interest, e.g. from primitive progenitors, intermediate precursors or mature osteoblasts. EST expression provides insight into possible interrelated physiological functions and putative interacting molecules during differentiation. This method offers a functional genomics approach to isolate differentiation stage-specific genes in samples as small as a single cell.

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A method is proposed for the derivation of a mathematical equation that relates the kinetic parameters of the propagation reactions of homogeneous free-radical chain reactions to the overall order of the reaction. The equation may be used in evaluating the predominant chain-termination reaction, provided that the mechanism of propagation of the chain is well understood. Application of the technique is demonstrated for the pyrolysis of n-butane. It predicts the predominance of the methyl-methyl radical recombination reaction.Canadian Journal of Chemistry, 47, 2707 (1969) Introduction Goldfinger, Letort, and Niclause (1) have shown that, for pyrolytic reactions, the overall order of the reaction depends on: (a) the order of initiation (first or second order), (b) the order of termination (second or third order), and (c) the type of radical participating in the chaintermination process. They referred to radicals involved in bimolecular propagation reactions as