This article describes the environmental impacts of producing a single seedling in forest nurseries of selected districts (i.e., Haripur, Abbottabad, and Mansehra) of Hazara Division of Khyber Pakhtunkhwa, Pakistan using the life cycle assessment (LCA) approach. This study was based on the cradle-to-gate approach which begins with the pre-nursery stage and progresses toward the main nursery before transplanting seedlings into the plantation site. Data or life cycle inventory (LCI) of seedling production were collected through questionnaire surveys and personal meetings with forest nurseries managers and workers regarding consumption of different inputs such as electricity, diesel, fertilizers, herbicides, and polyethylene bags, organic manure, and water consumption. The SimaPro software version 8.5 and the CML2000 v2.05 environmental model was applied to perform life cycle impact assessment (LCIA) for a single seedling production in forest nurseries in the study area. In line with the objectives of the study, primary data regarding inputs and outputs of the nurseries were collected from 35 nurseries in the study area by using a random questionnaire method. In addition, secondary data were taken from online databases such as Eco-invent v.3.2 CORRIM and peer-reviewed published literature. For this study, a functional unit of a single seedling was considered. Production weighted average data were modeled in the latest environmental modeling software i.e., SimaPro v.8.5 for ten US-EPA most wanted environmental impacts, such as global warming potential (GWP), abiotic depletion (AD), eutrophication potential (EP), acidification potential (AP), freshwater aquatic eco-toxicity (FAE), marine water eco-toxicity (MWE), terrestrial eco-toxicity (TE), ozone layer depletion (OLD), photochemical oxidation (PO), and human toxicity (HT). The results showed that the highest environmental impact posed by a single seedling was marine aquatic eco-toxicity (11.31360 kg 1,4-DB eq), followed by global warming potential (0.02945 kg CO2 eq) and (0.01227 kg 1,4-DB eq) human toxicity. The primary reason for these environmental burdens was the use of synthetic fertilizers in forest nurseries and the consumption of fossil fuels in nursery mechanization and transportation activities. The total cumulative energy demand for a single seedling was (0.800 MJ) with more than 90% contribution from fossil fuel energy resources such as petrol and diesel. It is therefore highly recommended to use renewable energy resources and organic fertilizers instead of chemical fertilizers in forest nurseries to avoid and minimize greenhouse gas emissions (GHS) and other toxic emissions in the study area.

1,4-alpha-D-glucan glucanohydrolase is among the most widely used commercial hydrolytic enzymes acting randomly on the glycosidic linkages of starch resulting in its saccharification and liquefaction. Its applicability in different industries can be improved by enhancing its stability and reusability. Therefore, in the present study attempts have been made to enhance the industrial applicability of 1,4-alpha-D-glucan glucanohydrolase from Bacillus subtilis KIBGE-HAR by adapting immobilization technology. The study developed mechanically stable, enzyme containing gel-frameworks using two support matrices including agar-agar, a natural polysaccharide and polyacrylamide gel, a synthetic organic polymer. These catalytic gel-scaffolds were compared with each other in terms of kinetics and stability of entrapped 1,4-α-D-glucan glucanohydrolase. In case of polyacrylamide gel, Km value for immobilized enzyme increased to 7.95 mg/mL, while immobilization in agar-agar resulted in decreased Km value i.e 0.277 mg/mL as compared to free enzyme. It was found that immobilized enzyme showed maximum activity at 70 °C in both the supports as compared to free enzyme having maximum activity at 60 °C. Immobilized 1,4-α-D-glucan glucanohydrolase exhibited no change in optimal pH 7.0 before and after entrapment in polyacrylamide gel and agar-agar. The enzyme containing gel-scaffold was found suitable for repeated batches of starch liquefaction in industrial processes. Agar-agar entrapped 1,4-α-D-glucanglucanohydrolase was capable to degrade starch up to seven repeated operational cycles whereas polyacrylamide entrapped enzyme conserved its activity up to sixth operational cycle.

A thorough life cycle assessment (LCA) was conducted to determine whether wood pellets were a viable substitute for non-renewable fuels like oil and gas, especially for heating. To evaluate the properties of wood pellets and their effects on the environment, the study was conducted in the Hazara division of Khyber Pakhtunkhwa, Pakistan. A few factors were investigated, including the carbon and water footprints and the identification of potential growth opportunities in the production of wood pellets. One kilogram of wood pellets served as the analysis reference unit. Raw materials were obtained from sawmills and furniture stores to make the wood pellets. Sawdust, a bio binder, and lubricating oil were used in the production process along with the pelletizer machine. SimaPro 9.2 software was used in the environmental footprint assessment to evaluate several environmental effects, including eutrophication, ozone depletion, abiotic depletion, rusting, human toxicity, and aquatic ecotoxicity. The highest contribution was shown by the wood pellets produced from the softwood sawdust as 149.8558 in marine aquatic ecotoxicity. The study’s findings showed that using lubricating oil during the production of wood pellets significantly affected the overall environmental results. The characterization of wood pellets showed that the Higher heating Values (HHV) resulted from burning wood pellets made from sawdust of Melia azedarach as 24.79 MJ/kg. Softwood mixed species recorded the highest water footprint and damage assessment impact and the highest carbon footprint of 0.186 CO2 e. With a 3.84 × 10−7 DALY (disability-adjusted life years) measurement, softwood mixed species showed the highest contribution to human health damage among the damage categories. In terms of cost, producing one kilogram of wood pellets from softwood mixed species was priced at 22 PKR, the lowest among the assessed species. The highest cost of 26 PKR was associated with producing wood pellets from Parthenium hysterophorus and Diospyros lotus.