Several indicators show that the world population is rapidly increasing with prospects of reaching close to 10 billion people in 2050. Optimal production of fish is a tentative goal to ensure human feeding and resources sustainability. The present study was performed on eggs of the silver carp, collected during the breeding season of consecutive years with different environmental temperature profiles at the Deroua Fisheries Station to evaluate the seasonal and inter-seasonal changes of eggs quality in order to promote a predictive marker for optimal production of silver carp. The viability of the obtained eggs was detected using ANOVA followed by the Tukey test to analyze fertilization rate, embryos survival rate, and fry survival rate. Data analysis showed no significant difference in the fertilization and survival rate of the embryos during the two breeding seasons, and therefore these can not be used as an adequate criterion to predict the viability of the fry of silver carp. Although the survival rate of the fry did not change during the first season, it changed significantly during the second when there was an increase in the environmental temperature. This factor could be responsible for the disturbance of the females' oogenesis and consequently the degradation of the eggs' quality. The results showed that the fry survival rate could be used as a parameter to predict the yield of silver carp production rather than the success of fertilization and the survival of embryos. This paper discusses the importance and scope of this approach.
The present study is performed in order to determine the relationship between fatty acid (FA) profile of silver carp (Hypophthalmichthys molitrix) eggs and fertilization success, embryos viability and larval production. Mature unfertilized ova were collected during the reproductive season from 23 mature and healthy females, reared in Deroua fish farm (Morocco). Total lipids were extracted from ova sample and subjected to trans-esterification then the resulting fatty acids methyl esters were analyzed by gas chromatography tandem mass spectrometry (GC/MS/MS). The results showed that silver carp ova are dominated on total FA by docosahexaenoic acid (DHA) (21.21%) followed by oleic acid (21.07%), palmitic acid (17.71%) and eicosapentaenoic acid (EPA) (10.25%). During the breeding season, polyunsaturateds (PUFAs), monounsaturateds (MUFAs) and saturated fatty acids (SFA) didn’t show any significant difference. Moreover, within the PUFAs, the n-3 series were more abundant than the n-6 series, the total mean was 31.57 ± 1.01% and 5.33 ± 0.32%, respectively. No correlation was between fatty acids and the fertilization success. Maternal weight has effect on the levels of oleic acid (C18:1), arachidonic acid (C20:4) and docosahexaenoic acid (C22:6) in the egg whereas levels of palmetoleic acid (C16:1), eicosapentaenoic acid (C20:5) and docosahexaenoic acid (C22:6) are related to female age.
Objective: To assess diagnostic accuracy of HRCT for COVID pneumonia keeping RT-PCR as reference standard. Study Design and Setting: A retrospective cohort study, carried out in of Radiology Department of KRL hospital, Islamabad from January 2021 to May 2021. Methodology: A total of 199 patients referred to Radiology Department for HRCT examination with clinical suspicion of COVID pneumonia were enrolled. Average age was 54yrs ± 14. PCR results of patients were retrieved from MIS. HRCT chest scan report assessed pulmonary involvement and categorized according to CORADS on a scale from 1 to 5. CT severity score was also assessed on 20-segment model for scoring. Statistical analysis was carried using SPSS software. Sensitivity, specificity, negative predictive value, positive predictive value and diagnostic accuracy were calculated. Results: The sensitivity was calculated to be 99.05%, the specificity was36.84%, the positive predictive value was 86.25%, the negative predictive value was 90.63% and diagnostic accuracy was 88.61%" For CORADS categories 1, 2 and 6, CT was in good agreement with the PCR results. Maximum numbers of patients were from age bracket 51-60 yrs. Correlation of gender with disease showed more prevalence in males and CTSS was not different in genders. Conclusion: HRCT chest has high sensitivity and negative predictive value for diagnosis of COVID pneumonia on the basis of CORADS reporting scheme. However it has low specificity. Disease has more prevalence in male gender. The most severely affected age bracket was 51-60 years.
Background: The lumbar spine's lumbrosacral transitional vertebrae (LSTV), a structural defect, have been variously linked to low back pain (LBP). Their presence can also lead to an incorrect pre-operative level identification. Objective: To examine the relationship between lumbosacral transitional vertebrae detected on plain radiographs and low back pain in patients. Study Setting: Department of orthopedics, Shifa international hospital, Islamabad Methods: The study design was prospective and descriptive. The study was conducted between July 2021 to December 2021. The radiographs of 100 patients with low back pain who met the inclusion/exclusion criteria were evaluated. The frequency of patients with low back pain and lumbosacral transitional vertebra (LSTV) was calculated using SPSS version 26. For the quantitative data presentation mean and standard deviation was utilized and for qualitative data frequencies were used. P value less than 0.05 was considered significant. Results: In a sample of 100 patients, 40 were men and 60 were women. 45 patients were diagnosed with axial type of lower back pain. The prevalence of LSTV was 27%, with males constituting the majority. No significant difference in the LSTV and Normal spine age distribution intervals was observed except, in the age interval of 51-60 years with a P-Value of <0.001. Conclusion: There is substantial evidence linking lumbosacral transitional vertebrae and low back pain.
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