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The essential oil of common juniper (Juniperus communis L., from the southern part of Serbia) and its fractions of different composition, as well as commercial antibiotics were used for testing their antimicrobial activity against bacteria, yeast and fungi. The essential oil was produced by hydro-distillation in a pilot plant (130 dm3) and then fractionated by distillation over a column, with 36 theoretical stages, under vacuum (26-66 mbar). The essential oil was also fractionated using pure CO2 or CO2 and methanol as co-solvent under supercritical conditions. The native oil showed weak antimicrobial activity, while the fractions with a high content of ?-pinene, and mixture of ?-pinene and sabinene showed the highest antimicrobial activity, especially against fungi. In comparison to the commercial antibiotics, the oil fractions showed more extensive spectra of antimicrobial activity, as well as wider inhibition zones. .

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