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Culture of Ruditapes decussatus is clearly limited by the availability of seed, as this production proceeds almost exclusively from natural recruitment. Artificial spawning and larval rearing programs could provide an alternative source of spat. This study was designed to evaluate the effect of different conditioning temperatures on the broodstock maturation, spawning success and larval viability of two geographically (north and south of the Iberian Peninsula) distinct populations of European clam (R. decussatus) collected at different periods of the year in order to create ''optimal'' artificial spawning and larval rearing programs. Two batches of clams from each population were collected in October and February, and conditioned at 18 ± 1°C, 20 ± 1°C and 22 ± 1°C. Of the three variables analysed the timing of broodstock collection was the most determining factor for gametogenic development, spawning and larval rearing. Geographic origin and conditioning temperature also greatly affected the spawning. The results also showed that the February conditioning was more effective than October and that the best conditioning temperatures were 20 ± 1°C and 22 ± 1°C for the northern and southern populations, respectively. These results suggest that the efficient conditioning temperature for each population of the same species is related to the seasonal temperature regime from their geographic origin. Larval viability and growth performance seemed to be independent of the broodstock conditioning.

Egg quality is one of the most important factors determining larval viability. The study of oocytes released by 'wild' broodstock will contribute to the definition of quality criteria applied to hatcheries as well as being an indicator of the oyster reproduction potential and aquaculture ongrowing systems. The aim of this study was to determine the relationship between condition of Crassostrea gigas spp. angulata (Portuguese strain) from 'wild' broodstock (condition index, gonadal maturity and number of oocyte released), oocyte biochemistry and hatching rate of eggs during the spring/summer of 1995. Stages of gonadal maturity and condition index indicated that natural spawning of this species occurred between April and August with the highest spawning intensity occurring in June. Analyses demonstrated that C. gigas oocyte organic matter consist of 44-74 % proteins, 16-38 % lipids and 7-12 % carbohydrates. A significant relationship was observed between some biochemical parameters-organic matter and lipid content-and broodstock condition index and hatching rate. It seems that these two oocyte parameters (organic matter and total lipids) could be used to define quality of oocyte and thus larval viability. The oocyte protein content was also found to be associated with broodstock and larval quality. In summary, these data are a contribution to the knowledge of the reproductive cycle of oysters in Europe and may be useful in improving hatchery management of this species.

► Characterize the reproductive cycle of two Portuguese populations of R. decussatus ► Describe the patterns of nutrient stored and utilization during gametogenic cycle ► The species showed a long spawning period that is useful to aquaculture production. ► R decussatus can adopt different reproductive strategies ► Contribute to establish a successful hatchery-based production of R. decussatus