Imobilisasi mikrokapsul minyak asiri jeruk nipis pada kain kapas telah dilakukan dengan metode pad dry cure menggunakan binder asam sitrat 3%. Minyak asiri dipreparasi menggunakan metode hidrodestilasi. Mikrokapsul berisi zat aktif alami minyak asiri jeruk nipis (C. aurantifolia) yang dibuat dengan metode koaservasi kompleks menggunakan penyalut alginat dan gelatin, pengikat silang glutaraldehid, dan pengemulsi polysorbate. Aktivitas antibakteri mikrokapsul sebelum diimobilisasi dan setelah diimobilisasi dilakukan terhadap bakteri Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, dan Klebsiella pneumoniae, dengan metode Kirby Bauer. Mikrokapsul dikarakterisasi dengan Particle Size Analyzer (PSA), Spektroskopi UV-Vis, dan Scanning Electron Microscopy (SEM). Hasil hidrodestilasi diperoleh rendemen minyak asiri sebesar 1,10%, berwarna kuning pucat, beraroma segar khas jeruk nipis. Hasil preparasi mikrokapsul diperoleh rendemen sebesar 46,28%; distribusi ukuran partikel homogen dengan ukuran pada rentang 1,604 µm; kandungan minyak 61,89%; efisiensi enkapsulasi 87,37%; dengan morfologi berbentuk bulat dengan permukaan halus. Kain yang telah diimobilisasi memiliki ketahanan cuci yang baik. Kain kapas tersebut dapat menghambat keempat bakteri S. aureus, S. epidermidis, E. coli, dan K. pneumoniae dengan daya hambat rata-rata berturut turut sebesar 12,
The Lime oil of Citrus aurantifolia was microencapsulated by coacervation technique employing gelatine and alginates as the shells, and calcium chloride as cross linker. Optimization of core/shell ratio, speed and temperature of stirring was carried out. Structure and shape of the microcapsules were characterized by particle size analyzer (PSA) and scanning electron microscope (SEM) respectively. The properties of microcapsules such as, yield, particle size distribution, oil content, oil load, and encapsulation efficiency were also determined. Upon condition of ratio core/shell 0.49, speed and temperature of stirring at 600 rpm and 35 �C respectively, the microcapsules of LOs with improved performance were achieved with efficiency of 46% and oil content of 78%.
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