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Pathologists play an important role in the final diagnosis for the patient and help the clinician in appropriate treatment planning. Both clinicians and pathologists have a crucial role in the laboratory process and it should be monitored properly for accurate and timely reporting.There are many steps in the laboratory process, where errors can occur which can affect the final diagnosis and prognosis for the patient. Errors can be major or minor but it eventually affects the patient's outcome. The laboratory process is broadly classified into three categories- pre-analytic, analytic and post-analytic phases. The errors that could frequently occur in each phase and the troubleshooting methods are broadly discussed in this article. Errors that have occurred should be investigated properly by identifying the main source of error and preventive action should be taken to avoid it in the future.

Oral Squamous Cell Carcinoma comprises nearly 90% of all Oral cancers. Recent studies on Oral Squamous Cell Carcinoma are done with a focus on identification of factors that affect the outcome of treatment, one of which is vitamin D levels. The aim of this study was to evaluate vitamin D3 levels in patients with Oral Squamous Cell Carcinoma and Normal Population with saliva as a biomarker. The study sample comprised of 40 subjects, of whom 20 were patients diagnosed with OSCC and 20 healthy subjects. The salivary samples obtained were assessed using Vitamin D3 ELISA kit. The mean salivary Vitamin D3 levels were 39.09 ng/dl among OSCC patients and 44.49 ng/dl among healthy subjects. Salivary Vitamin D levels in OSCC patients were found to be significantly less compared to the healthy controls. More than 90% of cancers of the oral cavity are Oral Squamous Cell Carcinoma (OSCC). Recent studies are done with a focus on identification of factors affecting the treatment of cancer, one of which is vitamin D levels. The aim of the study was to evaluate salivary vitamin D3 levels in patients with Oral Squamous Cell Carcinoma and in Normal Population. 40 patients were selected for the study. Salivary samples collected were assessed using Vitamin D3 ELISA Kit-EDI Total 25-OH Vitamin D EIA Kit to detect the salivary vitamin D levels. Mean Salivary Vitamin D3 levels were found to be 39.09 ng/dl in OSCC patients and 44.49 ng/dl in healthy subjects. A salivary Vitamin D level in OSCC patients was found to be significantly less compared to the healthy controls. Decreased Vitamin D3 levels in patients with OSCC indicate that Vitamin D3 deficiency increases the rate of cell proliferation, invasion, angiogenesis & metastasis in patients with OSCC. Vitamin D level is likely to reduce the severity of the disease and hence Vitamin D supplementation for OSCC patients in the early stages of treatment and after treatment can be done to enhance the prognosis.

Paget’s disease (PD) is a chronic bone disease characterised by increased resorption followed by excessive bone formation resulting in bone pain and fractures. PD is most common in the elderly and many are initially asymptomatic but can develop severe complications as disease progresses. Serum alkaline phosphatase is an important biochemical marker which is elevated in these patients. A clinical lion like face and radiological “cotton wool appearance” and a “jigsaw puzzle” pattern on histopathological examination are the characteristic features of PD. Bisphosphonates are the most commonly used drug of choice for treatment of patients with Paget’s disease. We discuss a classic case of PD involving a 70 year old female in this case report.

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