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In addition to their roles in protein biosynthesis, components of cellular ribosomes perform roles that contribute to a number of important cellular processes. Exploitation of processes has led to the use of ribosomal parts as solubility enhancer partners and purification matrices in protein expression. In this work, an engineered version of the E. coli ribosomal protein L29 (L4H2) as a fusion partner for enhancing cellular expression of proteins that are poorly expressed in bacteria was exploited. It was demonstrated that a chimeric fusion of L4H2 with various Fcγ receptors increases total expression up to 3.2-fold, relative to Fcγ receptors expressed without the fusion. Mechanistic insights using a novel application of in vivo ribosome display suggested that, although total cellular mRNA levels of L4H2-Fcγ receptor remained unchanged relative to wild-type Fcγ receptors, mRNA levels of actively translated L4H2-Fcγ transcript increased about 3.8-fold relative to actively translated levels of wild-type Fcγ transcript. Similar increases in protein expression in the context of the other proteins tested, showing the generality of this approach for proteins beyond human receptors was observed. These results extended the number of potential schemes by which orthogonal ribosomal parts can be used to enhance complex protein expression in bacterial platforms. Within a larger scope, this study features the possibility of engineering 5' tags that enhance mRNA affinity to ribosomes as strategies to augment translation. It was envisioned that the successful application of profiling active ribosomes in a highly targeted manner could be beneficial for mechanistic translation studies concerning synthesis of target proteins. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:285-293, 2016.

In this paper, we propose the streaming server Prior to software streaming technology, there exists Remote architecture for massive users and verify it by a small scale Execution which provides a user on a client access to remote simulation with virtual users. Software streaming is one of the applications [3] [4]. In detail, the applications are stored on a ASP solution that allows the execution of stream-enabled different machine and the local machine does not store or software, even while the transmission of the program may still be cache the applications. Microsoft's Terminal Service is one of in progress.the ASP solution based on thin client concept [5]. Clients justIn this paper, we analyze workload characteristics of the software stream, and extract four requirements that software receivesmoving pictresof exeutingapplications,uand most of streaming system have to satisfy. Based on these requirements, the processing is done on aremote server. Kuacharoen et al. [1] we design the software streaming server architecture. The presented the streaming architecture and method based on rich verification of the designed server is performed by virtual user client that most of the processing is done on the client. simulation. The result tells us the capacity of each server and Researches on workload characteristics of software stream helps us to calculate the scale of the system for massive users. and its influence on the performance of the server are at the initial stage. Because analyzing characteristics of traffic helps

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A simulator was developed to calculate the optical effects for OLED(Organic Light Emitting Devices). The optical effects for the bottom emitting structure and the top emitting structure were calculated with the various thickness of organic layer. The characteristic behavior of each organic layer was analyzed for these two structures. The optimal layer structures for these device structures were obtained.