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The seed oils of Persea americana and Melia dubua species are selected for this analytical study. These seed species yield 63.6 and 28 % of nonedible oil, respectively. The details of component fatty acids (CFAs) are collected from the literature. The necessary analytical data, viz., iodine value (IV) and saponification value (SV) of seed oils and major fuel properties of biodiesel, viz., cetane number (CN), higher heating value (HHV), and lower heating value (LHV) of the fatty acid methyl esters (FAMEs) are empirically computed. These parameters of biodiesel of seed oils under investigation are compared and evaluated in reference to existing biodiesels. This work reports the suitability of especially Persea americana for the biodiesel production.