A very simple, precise, economical, accurate, robust, and reproducible reverse phase-high-performance liquid chromatography method along with stability indicating attributes has been developed for estimating of prucalopride succinate (PRU) in both bulk and tablet formulation (PRUVICT 2). The estimation of the solutes was performed on a Grace C18 column of dimension 150 mm × 4.6 mm, 5 μm. PRU was eluted with acetonitrile: 0.02 M potassium dihydrogen phosphate in the ratio of 20:80 v/v in a 10 min isocratic mode at a flow rate of 1 ml/min at 30°C column temperature and monitored at a wavelength of 277 nm. The retention time of PRU was found to be 5.416 minutes. The Q2b validation of the analytical method revealed good linearity over the concentration range 2–12 μg/mL for IVA with r2 of 0.999. The mean recovery % over the three tested ranges of 50%, 100%, and 150% were found to be 100.173%, 99.077%, and 98.575%, respectively. In intra-day variability study, the % RSDs was detected to be 0.754, 1.032, and 0.482 whereas the inter-day variability study demonstrated % RSDs of 0.797, 0.559, and 0.524, respectively. The acid, alkali, boiled water, hydrogen peroxide, dry heat, and UV radiations based stress studies presented the formation of a variety of characteristic degradation products. The developed analytical method may be employed for the routine analysis of PRU in bulk and tablet formulations.

Presently, the research using Zebrafish is expanding into areas such as pharmacology, clinical research as a disease model and interestingly in drug discovery. Mammalian models of absorption, distribution, metabolism and excretion (ADME)/pharmacokinetics and efficacy are expensive, laborious and consume large quantities of precious compounds. There is also increasing pressure to limit animal use to situations in which they are absolutely necessary, such as in preclinical toxicity and safety assessment. The use of Zebrafish in pharmaceutical research, drug discovery and development is mainly target screening, target identification, target validation and drug toxicity study. Zebrafish have recently entered the fray as a model animal for some human diseases. It has numerous attributes in toxicology studies and high throughput screening. The fish are more affordable, easier to keep, and faster to rise than mammals, giving a higher-throughput system. Perhaps surprisingly, genes that cause disease in zebrafish are similar to those in humans. Zebrafish being a non-mammalian, drugs can also be tested for toxicity and their potential therapeutic activity against the target more easily than in mammals. The Zebrafish embryo has become an important vertebrate model for assessing drug effects. It exhibits unique characteristics, including ease of maintenance and drug administration, short reproductive cycle and transparency that permits visual assessment of developing cells and organs. Using Zebrafish it is possible to obtain results quickly at lower costs. "Reducing failures early in development is far more important than filling a pipeline with poorly chosen late-stage products likely to fail, and fail expensively."

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The present study aimed at preparing fast dissolving oral films of Eletriptan as a model drug which is used for the migraine treatment. Fast dissolving dosage forms have acquired great importance in pharmaceutical industry because of their unique properties. In the present research work various trials were carried out using different grades of HPMC E3, E6, and E15, maltodextrin DE6 and other polymers by solvent casting method. The prepared films were evaluated for film thickness, folding endurance, surface pH, morphological properties, % drug content and content uniformity, tensile strength, percent elongation, in vitro disintegration time and in vitro dissolution studies. The optimized formulation F24 prepared using HPMC E15 showed minimum disintegration time (10 sec), highest dissolution rate i.e. 99% of drug within 8 min and satisfactory physicochemical properties. The optimized film was evaluated for its bioavailability compared with pure drug as reference standard. Statistical analysis revealed that no significant difference between the bioavailability parameters of the film and the reference standard indicated that they exhibited comparable plasma level-time profiles. These findings suggest that the fast dissolving film containing Eletriptan is considered to be potentially useful for the treatment of migraine where quick onset of action is desirable.

The main objective of this study is to determine the concentration of trace elements in selected medicinal plants which are used for the treatment of dermal diseases. The trace element analysis was carried out in Trigonella foenum-graecum, Azadirachta indica, Vitex nigundo, and Argemone mexicana, using ED-X-ray Florescence (XRF) technique. The experiments were carried out using 3MV pelletron accelerator. The elements K, Ca, Cr, Mn, Fe, Cu, Zn, Rb, Sr., and Pb were identified in the sample. The elements K and Ca were present in maximum quantities. The relative concentrations of different elements in these medicinal plants have been given. All four plants contain trace elements of different concentrations in medicinal plants selected for this study. It is believed that the various trace elements present in the plants play an important role in the management of skin diseases. The present information will be helpful to prescribe the type of extract, dose, and mixture of these plants. The results justify that usage of these plants in the traditional systems of medicine for the treatment of skin diseases contains appropriate amounts of Fe, Zn, Cu, etc.