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histoquímica de los órganos subterráneos de plantas trepadoras medicinales rioplatenses (Argentina)". Lilloa 54 (2). El objetivo de este estudio fue caracterizar los rizomas y raíces por sus atributos histológico-estructurales y determinar la presencia de compuestos químicos que faciliten la identificación de los taxones de muestras enteras o fragmentadas de los mismos. Se analizó material fresco recolectado en el área de estudio y ejemplares de herbario (LPAG). Para las preparaciones anatómicas se procedió con técnicas usuales para microscopía óptica. Se realizaron reacciones histoquímicas para identificar almidón, mucílagos, lípidos y taninos. Los principales rasgos micrográficos resultaron: las características de las células y número de estratos celulares de la rizodermis y exodermis; el desarrollo de la peridermis; las peculiaridades del parénquima cortical y de la estela; el engrosamiento de las paredes celulares de las células corticales adyacentes a la endodermis y de la endodermis; la presencia de reservorios secretores y tipos de cristales. Sobre la base de los datos obtenidos se generaron tablas comparativas con los grupos químicos identificados y construyeron claves dicotómicas de las especies que constituyen una herramienta de valor en el control de calidad botánica.
Background and aims: Baccharis notosergila is an aggressive weed inhabiting the Salado river basin, Buenos Aires province, Argentina. The aims of this work were: to analyze the morpho-anatomy and histochemistry of aerial vegetative organs in order to understand the adaptation strategies that ensure its survival, as well as to expand knowledge on traits determining resistance to the control methods applied.
M&M: The material collected was prepared and examined with conventional techniques of microscopy. Histochemical tests to identify starch, resins, polyphenols, and lipophilic substances were performed.
Results: The major features found were small and deciduous leaves; uniseriate epidermis with massive and striate cuticle; stomata at level or slightly above the other epidermal cells and glandular trichomes secreting oily substances; stomata on both surfaces and isobilateral mesophyll. Tannins, starch and lipophilic substances were identified in leaves and stems; polyphenols, resins and lipids in ducts, and calcium oxalate crystals in leaves, stems and capitate trichomes.
Conclusions: The aerial vegetative organs features of B. notosergila explain its tolerance to the unfavorable conditions of the Salado river basin area, as well as its high competitive ability over others species of the natural prairie. The reduced and deciduous leaves, the epidermal traits, and chemical substances found constitute a physical and chemical barrier reducing dehydration as well as the penetration of the herbicides applied for its control. Botanical knowledge of B. notosergila is the basis for the design and development of new and appropriate management methods for this species.
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