The study area is home to many ethnic groups, civilizations, and geo-climatic conditions that support a wide range of plant species. Ethnomedicinal practices are widespread in this region, as ethnic people in the countryside rely significantly on medicinal herbs for their primary health care. An ethnomedicinal expedition and phytochemical screening of selected plants was conducted in Jhule-2 of Dolakha district, Nepal. The present study was carried out by a questionnaire based on interviewing local people of different age groups and laboratory analysis respectively. A total of 101 species of plants belonging to 60 families and 92 genera have been documented. The documented plants were used to treat various human ailments like gastro-intestinal, respiratory, dental, dermatological, orthopaedical disorder, snake bite, rheumatism, heart pain, headache, fever and other miscellaneous. Most of the extracts were prepared from leaves and twigs followed by fruit and seed. We observed a positive correlation (Pearson correlation, R 2 = 0.854) between the age of respondents and their knowledge regarding medicinal plants. For phytochemical screening, Gaultheria fragrantissima, Rhododendron arboreum and Taxus wallichiana were taken. Methanol extract from the plant samples was used for analyzing phytoconstituents like alkaloid, tannin, reducing sugar, glycosides, terpenoids, volatile oils, steroids, saponins, and flavonoids. The result showed the presence of phytoconstituents like tannin, flavonoid, terpenoid, alkaloids, and steroids. Reducing sugar was absent in T. wallichiana. Glycoside was absent in R. arboreum. The presence of volatile oil was shown only by T. wallichiana and G. fragrantissima. The present study reveals the use of medicinal plants for different ailments and nutritional purposes, and phytochemical analysis of those plants is recommended, which has not been tested yet.
