Background: Teamwork improves self-reporting efficiency on inter-professional practice. It can also increase the self-confidence, awareness, and the ability of members to manage high-risk situations. The aim of this study was to translate and assess psychometrically the attitudes toward healthcare teamwork scale; adopted from previous studies. Methods: This cross sectional study consisted of 200 students who were recruited from the nursing and medical schools in Tehran. Convenience sampling method was used to select subjects. Initial instrument comprised 15 items with Likert type response. The impact score, content validity index (CVI) and content validity ratio (CVR) were assessed and an exploratory factor analysis (EFA) was used to extract factors. Confirmatory factor analysis was used to confirm the factorial structure of the questionnaire using SPSS (version 19) and Amos (version 23), respectively. The reliability of the questionnaire was calculated using Cronbach's alpha coefficient and test-re-test. Results: Eleven items were considered valid on the basis of impact score of more than 1.5, CVR above 0.62, and CVI above 0.7. The exploratory factor analysis deemed all the 11 items valid. With respect to the eigenvalue above one for each factor, three factors were extracted. This instrument had 55% of variance in the attitude toward healthcare teamwork scale. Conclusions:The results of this study are a suitable evidence on the strength of the factor structure and good reliability for the psychometric property of the attitudes toward health care teamwork scale. The results of this study can be used by education centers and institutes to assess student's attitudes towards healthcare teamwork.