We document the first record of Laval’s Disk-winged Bat, Thyroptera lavali Pine, 1993, from the Amazon region of Colombia. This record increases to four the number of Thyroptera species in the country and extends the known range of T. lavali 428 km east and 338 km north from the nearest previous localities. The distribution of this species now comprises 11 localities from six ecoregions and five countries: Brazil, Colombia, Ecuador, Peru, and Venezuela. We highlight the paucity of knowledge on bat species in the Colombian Amazon.