Objectives: The aim of this study is to evaluate the effect of Platelet Rich Fibrin (PRF) in bone regeneration and healing in cases of fracture mandible. Patients and Methods: Forty patients diagnosed clinically and radiographically with mandibular fracture treated with open reduction and rigid internal fixation, they were randomly divided into two groups (n=20): Group A; the study group (Fixation with plates and screws with PRF membrane along the fracture line) and Group B; the control group (Fixation with platelet and screws only). Mean Bone Density was assessed via Computed tomography at 1week, 3 months, 6 months postoperative. The pain was assessed using numerical rating scale (NRS) during 1st, 3rd day and 1 week postoperatively, as well as maximum mouth opening. Results: Increased mean bone density was observed in the study group which was statistically significant. The mean bone density for the study group at 1week, 3 months and 6 months were 270.80±79.60, 575.40±83.49 and 890.10±130.55 respectively. In contrast to control group, the mean bone density was of 223.00±53.53, 408.80±76.59 and 635.80±94.58 for 1 week, 3months and 6 months respectively. There was no statistically significant difference as regard pain and maximum mouth opening between both groups. Conclusion: PRF application as a biologic surgical additive at the fracture line in mandibular fractures promotes early bone regeneration and soft tissue healing due to the presence of growth factors.