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Detection of endangered species is invaluable for conservation efforts, yet many traditional sampling techniques are ineffective at low population abundances or during certain periods of the year. Here, we compared results from a newly developed eDNA approach and the traditional observational method for the endangered pool frog (Pelophylax lessonae). Analysis using an occupancy-modeling framework indicated that the probability of pools being occupied using eDNA (0.93) was higher than for the traditional method of counting calling males and silent observed individuals (0.72). Detailed analysis revealed complementarity among the methods. That is, the traditional method gave a high rate of observation in June, whereas eDNA gave at least as many or more observations during other parts of the year. Discrepancies among the methods depended on the dominant lifecycle stage, and eDNA concentrations were higher when juveniles were present than at times when spawning occurred. eDNA concentrations were also positively related to P. lessonae observations. Our study demonstrates that an eDNA protocol for monitoring of endangered amphibian species can be particularly valuable during periods when individuals are hard to detect by observational methods, and provides guidance to sampling efforts for research and monitoring programs in other regions and systems.

Abstract. Global warming is expected to be most pronounced in the Arctic where permafrost thaw and release of old carbon may provide an important feedback mechanism to the climate system. To better understand and predict climate effects and feedbacks on the cycling of elements within and between ecosystems in northern latitude landscapes, a thorough understanding of the processes related to transport and cycling of elements is required. A fundamental requirement to reach a better process understanding is to have access to high-quality empirical data on chemical concentrations and biotic properties for a wide range of ecosystem domains and functional units (abiotic and biotic pools). The aim of this study is therefore to make one of the most extensive field data sets from a periglacial catchment readily available that can be used both to describe present-day periglacial processes and to improve predictions of the future. Here we present the sampling and analytical methods, field and laboratory equipment and the resulting biogeochemical data from a state-of-the-art whole-ecosystem investigation of the terrestrial and aquatic parts of a lake catchment in the Kangerlussuaq region, West Greenland. This data set allows for the calculation of whole-ecosystem mass balance budgets for a long list of elements, including carbon, nutrients and major and trace metals. The data set is freely available and can be downloaded from PANGAEA:

In order to assess the potential radiological risk to humans and the environment from a geological repository for radioactive waste, a safety assessment must be performed. This implies that the release and transfer of radionuclides from the repository into the surface environment are calculated and that the effects in the biosphere are evaluated for an assessment period up to one hundred thousand years according to Swedish regulations. This paper discusses the challenges associated with the modelling of surface ecosystems over such long time scales, using the recently completed assessment for the extension of the existing repository for the low- and intermediate-level nuclear waste (called SFR) in Forsmark, Sweden as an applied example. In the assessment, natural variation and uncertainties in climate during the assessment period were captured by using a set of climate cases, primarily reflecting different expectations on the effects of global warming. Development of the landscape at the site, due to post-glacial isostatic rebound, was modelled, and areas where modelling indicated that radionuclides could discharge into the biosphere were identified. Transfers of surface water and groundwater were described with spatially distributed hydrological models. The projected release of radionuclides from the bedrock was then fed into a biosphere radionuclide transport model, simulating the transport and fate of radionuclides within and between ecosystems in the landscape. Annual doses for human inhabitants were calculated by combining activity concentrations in environmental media (soil, water, air and plants) with assumptions on habits and land-use of future human inhabitants. Similarly, dose rates to representative organisms of non-human biota were calculated from activity concentrations in relevant habitats, following the ERICA methodology. In the main scenario, the calculated risk for humans did not exceed the risk criteria or the screening dose rate for non-human biota, indicating that the repository design is sufficient to protect future populations and the environment. Although the combination of radionuclides, land-uses/habitats, type of most exposed population and area of exposure that contribute most to the total dose shifts over time, the total calculated dose shows limited variability. Significant reductions in the dose only occur during submerged periods and under periglacial climate conditions. As several different water and food pathways were equally important for endpoint results, it is concluded that it would be difficult to represent the biosphere with one or a set of simplified models. Instead, we found that it is important to maintain a diversity of food and water pathways, as key pathways for radionuclide accumulation and exposure partly worked in parallel.

To assist transport modeling in assessments of the radiological impact of a geological repository for radioactive wastes, the mobility of various elements was studied in arable and wetland soils in the Forsmark region, Sweden. Pore water and total element contents were determined for five types of unconsolidated deposits (regolith), spanning a wide range of soil properties with respect to pH and organic matter content. Two soil depths were sampled to capture element mobility in regolith layers affected and unaffected by soil-forming processes. The solid/liquid partition coefficients (Kd values) for most elements varied significantly among regolith types. For most elements, the observed variations in Kd values could be explained by variations in soil properties. For many elements, mobility increased with decreasing soil pH. The results provide a significant addition of data on radionuclide retention in soils, taking account of soil properties and processes.Electronic supplementary materialThe online version of this article (doi:10.1007/s13280-013-0408-4) contains supplementary material, which is available to authorized users.