Collagen methacrylation is a promising approach to generate photo-cross-linkable cell-laden hydrogels with improved mechanical properties. However, the impact of species-based variations in amino acid composition and collagen isolation method on methacrylation degree (MD) and its subsequent effects on the physical properties of methacrylated collagen (CMA) hydrogels and cell response are unknown. Herein, we compared the effects of three collagen species (bovine, human, and rat), two collagen extraction methods (pepsin digestion and acid extraction), and two photoinitiators (lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and Irgacure-2959 (I-2959)) on the physical properties of CMA hydrogels, printability and mesenchymal stem cell (MSC) response. Human collagen showed the highest MD. LAP was more cytocompatible than I-2959. The compressive modulus and cell viability of rat CMA were significantly higher (p < 0.05) than bovine CMA. Human CMA yielded constructs with superior print fidelity. Together, these results suggest that careful selection of collagen source and cross-linking conditions is essential for biomimetic design of CMA hydrogels for tissue engineering applications.
In new residential structures and green architecture, it is necessary to maintain the heat of the internal environment to an appropriate level throughout winter conditions with low electricity usage. This work is thus intended to produce environmentally acceptable isolation substances (organic material). Lignocellular biomass, which is also referred to as Poaceae common reed and Phragmites australis and straw, were used as organic material in this study. During testing of its performance under controlled settings, the insulating effectiveness of these organic compounds was assessed. The exploratory project comprises three forms of isolation: organic made from straw and reed, industrial isolation (fibreglass), and brickwork without insulation. An infrared sensor was used to calculate the quality of isolation. For each isolation situation, the temperature characteristic was produced. The findings show that fibreglass was equivalent to the effectiveness of the organic isolation. Furthermore, the efficiency difference was 0. 84 percent comparing the industrial and organic isolation substances, which shows that Lignocellusic Biomass is a viable environmental-friendly replacement to industrial isolation substances.
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