Disorders of perineal musculature, as the bulbospongiosus muscle (Bsm), could cause male sexual dysfunctions, such as premature ejaculation. We determined the effect of sucrose water consumption during pregnancy-lactation and postweaning on the Bsm frequency during the urethrogenital reflex (UGR), which simulates erection and ejaculation, in adult male rat offspring. Twelve female rats were mated and grouped in control mothers consumed tap-water and sucrose mothers consumed sucrose-water during pregnancy-lactation. Male pups were weaned and assigned into four groups (n = 12 each): those from control mothers who continued drinking tap-water (CM–CO group) or sucrose-water (CM–SO group) until adult life, and those from sucrose mothers who drank tap-water (SM–CO group) or continued drinking sucrose-water (SM–SO group) until adult life. We analyzed Bsm electromyograms during penile stimulation and UGR in male rat offspring (n = 6; each group). We analyzed the frequency of the Bsm through Fourier Transform. Other males rat offspring (n = 6; each group) were killed and was collected the Bsm and processed into histological analysis. Two-way ANOVA followed by posthoc tests, considering P ≤ 0.05 significant difference. The results showed that the consumption of sucrose-water during critical stages increases significantly the frequency of Bsm during the UGR of the CM-SO, SM-CO and SM-SO. Also, the consumption of high-sugar diets changes the morphological arrangement of the Bsm. Our results suggest that a combination of a high-sugar diet during pregnancy, lactation, and post-weaning changes the Bsm morpho-physiology and could cause male sexual dysfunctions.