Office workers have insufficient physical activity ( PA) and high amount of sedentary behavior ( SB) . Several studies reported the association between PA, SB, and abdominal obesity in health adults. They also found that abdominal obesity was associated with poor cognitive function. However, less number of study determine the association between PA, SB, body composition, metabolic profiles, and cognitive function in office workers. The objective of this study was to study the association between PA, SB, body compositions, metabolic profiles, and cognitive function in office workers. A total of 22 office workers were recruited from Faculty of Physical Therapy, Mahidol University. Data were age, gender, body mass index (BMI), waist to hip ratio (WHR), and waist to height ratio (WHtR). Moderate to vigorous PA and duration of performance were recorded. SB was the amount of sitting time during work, leisure activity, and commute. The percentage of body fat, muscle mass ( kg) , and basal metabolic rate were measured by the bioelectrical impedance analysis (BIA). We used the data of annual medical check-up data for metabolic profiles (i.e., LDL, HDL, and fasting blood glucose). Subjects were assessed four domains of cognitive function including memory, language, executive function, and information processing speed domains. Data were analyzed using Spearman's rank correlation. Our results demonstrated that amount of sitting time during leisure, body composition, LDL, and total cholesterol were negative correlated with cognitive function including language and executive domains. High calories expenditure was reported in overweight/obese compared to normal. Future study should investigate the amount of sitting time influenced on metabolic profiles and cognitive function.