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In this study, pretreated organic wastes such as waste paper cups, cardboard waste, and vegetable waste were screened for the growth and lipid production of oleaginous bacteria DS-7 (isolated from the dairy effluent scum). The pretreated vegetable waste was found to be the best feedstock for biomass and lipid production by the DS-7. Further, process parameters such as inoculation time, substrate concentration (w/v) (amount of pretreated vegetable waste), pH, and inoculum size were optimized using a multi-objective optimization technique to enhance the biomass and lipid productions. The optimization study successfully enhanced the biomass concentration (g/L) and lipid content (%) by 47.9% and 15.84%, respectively in comparison with the unoptimized state. The biomass and lipid productivities were 42% (1.449 g/L/d) and 51% (1.267 g/L/d) greater than unoptimized conditions. The characteristics of the biodiesel obtained from the valorization of vegetable waste were comparable to the standard. Thus, the vegetable waste can be utilized as a potential feedstock for microbial biodiesel production.

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