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A transplantable tumor of inbred mice was obtained by inoculating BALB/c mice subcutaneously with SV40‐transformed mouse kidney (mKS‐A) cells. Tumors were produced by mKS‐A cells in the 71st cell culture passage, but not by cells in the 26th passage. The tumor line has been serially passed in BALB/c mice 14 times. In vitro cell culture lines were derived from tumors after 1, 2, 8, 10 and 12 passages in mice. The tumors, as well as the In vitro tumor cell lines, contained SV40 T‐antigen, and sera from the tumor‐bearing mice contained antibodies to the SV40 T‐antigen. SV40 was rescued from the In vitro tumor cell lines after fusion with green monkey kidney (CV‐1) cells in the presence of UV‐irradiated Sendai virus. The In vitro tumor cell lines derived from mouse passages 8, 10 and 12 were used as SV40 virus; 2) SV40‐transformed cell lines; 3) primary mouse (BALB/c or Yale Swiss) kidney cells, or 4) primary mouse (BALB/c or Yale Swiss) embryo cells. These results showed that the tumor line and the In vitro tumor cell lines have the transplantation antigen.