Background: Tetracycline class of antibiotics differ in their pharmacokinetic profile and chelating property. Objective of present study is to assess the effect of oxytetracycline and doxycycline on calcium chelationMethods: For estimation of calcium chelation of Oxytetracycline and Doxycycline, EDTA method (P. Trinder) and calcium binding assay was followed. Different doses of Oxytetracycline (25 mg, 50 mg and 100 mg) and Doxycycline (25 mg, 50 mg and 100 mg) were used in EDTA method and different concentrations of calcium were used in calcium binding assay. The procedure was done according to the standard methodology.Results: The intensity of colour appear to be increased with increase in dose of the Oxytetracycline (25 mg, 50 mg, 100 mg) as the concentration of calcium binding increases. But in Doxycycline intensity of colour is more with 100 mg as compared with 25 mg and 50 mg The UV absorption spectrum of solution of Oxytetracycline (1mM) was changed after the addition of CaCl2 to provide different concentration of Ca2+ (0.1, 0.5 and 1.0 mM).  With minor shift in the absorption coefficient and no shift in wavelength were observed for Doxycycline.Conclusions: The study concludes that oxytetracycline has more calcium chelating property than doxycycline.

Background: There is a shift in the teaching learning process with the advent of newer methodologies. The present study was conducted to obtain feedback from 2 year dental students regarding teaching and learning methodology and their perception about pharmacology.Methods: This was an observational, cross-sectional questionnaire based survey. A self-designed questionnaire containing 10 questions with 4-6 options regarding the teaching and learning methodology was given to 2nd year dental students. Responses was collected and data was compiled in Microsoft excel and statistically analyzed.Results: A total of 97 students participated in this survey. Students opinion revealed that, Gastrointestinal followed by general pharmacology and cardiovascular system were the most favorite topics. Majority preferred studying from “standard text books” and method of learning was “understanding” of the subject rather mugging. Majority of the students said that preferred lectures taken via LCD projectors and rated viva and MCQ as preferred method of evaluation university exams. Interactive classes and tutorials were the most preferred techniques.Conclusions: The study concluded that interactive classes and overhead /LCD based lectures should be inculcated in routine learning and teaching program of pharmacology. There is a need to qualitatively assess the various teaching and learning methods, so as to understand students views and their perception for improving teaching and learning pharmacology. The outcome will help us to modify and improve quality of under graduate dental pharmacology teaching and learning.

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