Main conclusion Bambara groundnut has the potential to be used to contribute more the climate change ready agriculture. The requirement for nitrogen fixing, stress tolerant legumes is clear, particularly in low input agriculture. However, ensuring that existing negative traits are tackled and demand is stimulated through the development of markets and products still represents a challenge to making greater use of this legume.

Using experience with bambara groundnut (Vigna subterranea), this paper examines how local knowledge, genetic evaluation, research in fields, glasshouses and laboratories, and crop simulation modelling might be linked within a methodological framework to assess rapidly the potential of any underutilized crop. The approach described is retrospective in that each activity was not clearly defined and structured at the outset. However, the experience gained may help to establish a methodology by which growers, researchers and international agencies can integrate their knowledge and understanding of any particular underutilized crop and apply similar principles to accelerate the acquisition of knowledge on other underutilized species. The use of a methodological framework provides a basis for activities that maximize knowledge, minimize duplication of effort, identify priority areas for further research and dissemination, and derive general principles for application across underutilized crops in general. It also allows policy makers and planners to make comparative decisions on the nutritional, economic and research importance of different underutilized and more-favoured species. In particular, the incorporation of a generic crop simulation model within the methodological framework may assist growers, extension agencies and scientists to refine general recommendations for any particular crop to local conditions. Also, the incorporation of information gathered from the field, laboratory or market can be used to update rapidly the predictive capacity of the model for each crop.

Genetic diversity of a Bambara groundnut germplasm representing accessions from 25 African countries, maintained at the International Institute of Tropical Agriculture (IITA; Nigeria) was evaluated based on seed patterns, qualitative characters, quantitative traits and Diversity Arrays Technique (DArT) markers. The study aimed at identifying important descriptors for germplasm conservation, validating the crop's geographical centre of origin and facilitating the utilization of existing genetic resources. Frequencies and diversity indices of seven types of seed patterns, 12 qualitative and 28 quantitative traits were evaluated for 124 representative accessions and were analyzed by region to elucidate the geographic distribution of descriptor states. In addition, individual plant samples from a subset of 40 landrace accessions were analyzed by assessing 554 DArT markers. Both the phenetic tree constructed from the qualitative and quantitative descriptors and the population structure derived from DArT marker analysis suggested a relatively high genetic diversity among accessions. Higher genetic diversity was observed for the Cameroon/Nigeria region relative to other regions, in agreement with the hypothesis that this region is the centre of origin for Bambara groundnut. Use of an extensive and diverse range of germplasm and an approach that integrated morphological and quantitative descriptors with DNA markers that represent wide genomic coverage offered a powerful way to make inferences about crop germplasm, in support of crop-improvement programs.

A number of novel strategies were employed to examine the role of indoleacetic acid (IAA) in regulating floral organ abscission in Arabidopsis (Arabidopsis thaliana). Analysis of auxin influx facilitator expression in b-glucuronidase reporter plants revealed that AUXIN RESISTANT1, LIKE AUX1, and LAX3 were specifically up-regulated at the site of floral organ shedding. Flowers from mutants where individual family members were down-regulated exhibited a reduction in the force necessary to bring about petal separation; however, the effect was not additive in double or quadruple mutants. Using the promoter of a polygalacturonase (At2g41850), active primarily in cells undergoing separation, to drive expression of the bacterial genes iaaL and iaaM, we have shown that it is possible to manipulate auxin activity specifically within the floral organ abscission zone (AZ). Analysis of petal breakstrength reveals that if IAA AZ levels are reduced, shedding takes place prematurely, while if they are enhanced, organ loss is delayed. The At2g41850 promoter was also used to transactivate the gain-of-function AXR3-1 gene in order to disrupt auxin signaling specifically within the floral organ AZ cells. Flowers from transactivated lines failed to shed their sepals, petals, and anthers during pod expansion and maturity, and these organs frequently remained attached to the plant even after silique desiccation and dehiscence had taken place. These observations support a key role for IAA in the regulation of abscission in planta and reveal, to our knowledge for the first time, a requirement for a functional IAA signaling pathway in AZ cells for organ shedding to take place.The shedding of plant organs plays a key role during the life cycle of a plant . It can limit the spread of systemic invasion by pathogens, provide a mechanism to remove damaged or inefficiently functioning tissues, remove competition for pollinators from fertilized flowers, and contribute to seed dispersal in dry and fleshy fruits (Leslie et al., 2007). The timing of flower and fruit abscission is a process of substantial interest to the horticultural and agricultural industries, as it can affect both the quantity and quality of yield. Indeed, the formation of an abscission zone (AZ) was one of the first traits to be manipulated during the advent of agricultural practices (Doebley, 2004). Considerable research interest, therefore, has been dedicated to identifying the endogenous and environmental factors that trigger the process and regulate the rate at which it proceeds.Research by Jackson and Osborne (1970) showed that ethylene was a natural regulator of abscission and that exposure to the gas hastened the shedding of leaves, flowers, and fruit. Prior to this discovery, it had been reported that the attachment of orchid (Dendrobium spp.) pollinia, known to be rich in auxin, to excised coleus tissue dramatically slowed abscission (Laibach, 1951) and that application of indoleacetic acid (IAA) to the distal end of bean (Phaseolus vulgaris) leaf explants delayed ce...

Rapid population growth, climate change, intensive monoculture farming, and resource depletion are among the challenges that threaten the increasingly vulnerable global agri-food system. Heavy reliance on a few major crops is also linked to a monotonous diet, poor dietary habits, and micronutrient deficiencies, which are often associated with diet-related diseases. Diversification—of both agricultural production systems and diet—is a practical and sustainable approach to address these challenges and to improve global food and nutritional security. This strategy is aligned with the recommendations from the EAT-Lancet report, which highlighted the urgent need for increased consumption of plant-based foods to sustain population and planetary health. Bambara groundnut (Vigna subterranea (L.) Verdc.), an underutilized African legume, has the potential to contribute to improved food and nutrition security, while providing solutions for environmental sustainability and equity in food availability and affordability. This paper discusses the potential role of Bambara groundnut in diversifying agri-food systems and contributing to enhanced dietary and planetary sustainability, with emphasis on areas that span the value chain: from genetics, agroecology, nutrition, processing, and utilization, through to its socioeconomic potential. Bambara groundnut is a sustainable, low-cost source of complex carbohydrates, plant-based protein, unsaturated fatty acids, and essential minerals (magnesium, iron, zinc, and potassium), especially for those living in arid and semi-arid regions. As a legume, Bambara groundnut fixes atmospheric nitrogen to improve soil fertility. It is resilient to adverse environmental conditions and can yield on poor soil. Despite its impressive nutritional and agroecological profile, the potential of Bambara groundnut in improving the global food system is undermined by several factors, including resource limitation, knowledge gap, social stigma, and lack of policy incentives. Multiple research efforts to address these hurdles have led to a more promising outlook for Bambara groundnut; however, there is an urgent need to continue research to realize its full potential.