Background and objective: Acinetobacter baumannii is a significant pathogenic bacterium in the health system. The ability to resist antimicrobial drugs and biofilm formation gives the considerable capacity to A. baumannii for existing in a harsh environment, enabling this bacterium to cause hospital-acquired infection. Carbapenem is an important treatment option for severe nosocomial infection and patients infected by multidrug-resistant organisms. The main aim of this study is to detect carbapenemase in isolates, and its association with biofilm formation as well as antibiotic resistance. Methods: Sixty A. baumannii isolates were obtained from several hospital districts in Erbil city. Identification and antimicrobial susceptibility test (AST) of isolates were performed by VITEKII compact system. Phenotypic identification of carbapenem by sCIM also biofilm-forming was detected by 96 well method. Additionally, three antimicrobial agents were used if they were successful in eliminating biofilm formation. . Results: The majority of the isolates were from sputum, accounting 75% and antibiotic susceptibility showed that the isolates are resistant to the most available antibiotics, and significant of the isolates formed strong biofilm. The sensitivity of meropenem, ceftazidime, and ciprofloxacin were employed for ten isolates of A. baumannii after biofilm formation it was found that biofilm cells need more concentration of antibiotic than planktonic cells then phenotypic detection of carbapenem showed that the overall positive values were 30 (50.0%) for sCIM. Conclusion: We revealed that most resistant isolates have a greater capacity for biofilm development than sensitivite isolates. Biofilm-producing strains of A. baumannii cannot be killed with the relatively similar concentration of antimicrobial drugs that are needed to kill planktonic cells.