Sub-acute toxicity profile of Rheumatic Tea Formula (RTF), a polyherbal tea consisting of Salix alba, Eucalyptus globulus and Albizia chevalieri was investigated in wistar rats of both sexes. Wistar rats were orally administered three different doses of ethanol extract of RTF for 28 days after which the effect on body weight, feed consumption, water intake, liver and kidney functions as well as haematological parameters were evaluated. Also evaluated was the histolopathogical effect of the extract on the liver, kidney, spleen and stomach. The extract significantly (p < 0.05) reduced body weight, feed consumption and water intake in rats. On liver function, there was no significant effect of Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST); however there was significant (p < 0.05) elevation of Alkaline Phosphatase (ALP). No significant effect (p > 0.05) on kidney function indices was observed. There was also no significant effect (p > 0.05) on haematological parameters, except for WBC differentials where there was significant (p < 0.05) elevation of lymphocytes and decrease in neutrophils. The extract was relatively non-toxic on kidney, liver, spleen, and stomach in the histopathological evaluations. The present study suggests that RTF extract might have no serious deleterious effect on sub-acute administration.