BackgroundStomatal behavior in grapevines has been identified as a good indicator of the water stress level and overall health of the plant. Microscope images are often used to analyze stomatal behavior in plants. However, most of the current approaches involve manual measurement of stomatal features. The main aim of this research is to develop a fully automated stomata detection and pore measurement method for grapevines, taking microscope images as the input. The proposed approach, which employs machine learning and image processing techniques, can outperform available manual and semi-automatic methods used to identify and estimate stomatal morphological features.ResultsFirst, a cascade object detection learning algorithm is developed to correctly identify multiple stomata in a large microscopic image. Once the regions of interest which contain stomata are identified and extracted, a combination of image processing techniques are applied to estimate the pore dimensions of the stomata. The stomata detection approach was compared with an existing fully automated template matching technique and a semi-automatic maximum stable extremal regions approach, with the proposed method clearly surpassing the performance of the existing techniques with a precision of 91.68% and an F1-score of 0.85. Next, the morphological features of the detected stomata were measured. Contrary to existing approaches, the proposed image segmentation and skeletonization method allows us to estimate the pore dimensions even in cases where the stomatal pore boundary is only partially visible in the microscope image. A test conducted using 1267 images of stomata showed that the segmentation and skeletonization approach was able to correctly identify the stoma opening 86.27% of the time. Further comparisons made with manually traced stoma openings indicated that the proposed method is able to estimate stomata morphological features with accuracies of 89.03% for area, 94.06% for major axis length, 93.31% for minor axis length and 99.43% for eccentricity.ConclusionsThe proposed fully automated solution for stomata detection and measurement is able to produce results far superior to existing automatic and semi-automatic methods. This method not only produces a low number of false positives in the stomata detection stage, it can also accurately estimate the pore dimensions of partially incomplete stomata images. In addition, it can process thousands of stomata in minutes, eliminating the need for researchers to manually measure stomata, thereby accelerating the process of analysing plant health.

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Please cite this article in press as: S. Liu, M. Whitty, Automatic grape bunch detection in vineyards with an SVM classifier, Journal of Applied Logic (2015), http://dx. AbstractPrecise yield estimation in vineyards using image processing techniques has only been demonstrated conceptually on a small scale. Expanding this scale requires significant computational power where, by necessity, only small parts of the images of vines contain useful features. This paper introduces an image processing algorithm combining colour and texture information and the use of a support vector machine, to accelerate fruit detection by isolating and counting bunches in images. Experiments carried out on two varieties of red grapes (Shiraz and Cabernet Sauvignon) demonstrate an accuracy of 88.0% and recall of 91.6%. This method is also shown to remove the restriction on the field of view and background which plagued existing methods and is a first step towards precise and reliable yield estimation on a large scale.

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