Lay SummaryAcute myeloid leukemia is a highly malignant hematopoietic tumor that affects about 13,000 adults yearly in the United States. The treatment of this disease has changed little in the past two decades, since most of the genetic events that initiate the disease remain undiscovered. Whole genome sequencing is now possible at a reasonable cost and timeframe to utilize this approach for unbiased discovery of tumor-specific somatic mutations that alter the protein-coding genes. Here we show the results obtained by sequencing a typical acute myeloid leukemia genome and its matched normal counterpart, obtained from the patient’s skin. We discovered 10 genes with acquired mutations; two were previously described mutations thought to contribute to tumor progression, and 8 were novel mutations present in virtually all tumor cells at presentation and relapse, whose function is not yet known. Our study establishes whole genome sequencing as an unbiased method for discovering initiating mutations in cancer genomes, and for identifying novel genes that may respond to targeted therapies.We used massively parallel sequencing technology to sequence the genomic DNA of tumor and normal skin cells obtained from a patient with a typical presentation of FAB M1 Acute Myeloid Leukemia (AML) with normal cytogenetics. 32.7-fold ‘haploid’ coverage (98 billion bases) was obtained for the tumor genome, and 13.9-fold coverage (41.8 billion bases) was obtained for the normal sample. Of 2,647,695 well-supported Single Nucleotide Variants (SNVs) found in the tumor genome, 2,588,486 (97.7%) also were detected in the patient’s skin genome, limiting the number of variants that required further study. For the purposes of this initial study, we restricted our downstream analysis to the coding sequences of annotated genes: we found only eight heterozygous, non-synonymous somatic SNVs in the entire genome. All were novel, including mutations in protocadherin/cadherin family members (CDH24 and PCLKC), G-protein coupled receptors (GPR123 and EBI2), a protein phosphatase (PTPRT), a potential guanine nucleotide exchange factor (KNDC1), a peptide/drug transporter (SLC15A1), and a glutamate receptor gene (GRINL1B). We also detected previously described, recurrent somatic insertions in the FLT3 and NPM1 genes. Based on deep readcount data, we determined that all of these mutations (except FLT3) were present in nearly all tumor cells at presentation, and again at relapse 11 months later, suggesting that the patient had a single dominant clone containing all of the mutations. These results demonstrate the power of whole genome sequencing to discover novel cancer-associated mutations.

Thin coatings of photoresponsive, pyrimidine-terminated molecules, attached to gold or quartz substrates in contact with water, undergo dimerization and wettability changes when irradiated with UV light at 280 and 240 nm. Self-assembled monolayers of long chain thymine-terminated thiols give the largest, reversible photoinduced contact angle changes. The latter are caused by a decrease in surface charge as the thymine monomer dimerizes upon irradiation, a process which is accompanied by an increase in the acidity constant of the dimer. Uracil self-assembled monolayers photodimerize but do not photocleave; there is an irreversible change in contact angle. Spin-cast films of thymines give smaller contact angle changes, the maximum values corresponding to films which are composed of a mixture of crystalline and amorphous states.

Here, translated into English, GENETICS republishes the original Mendel article. As discussed in the Perspectives by Daniel J. Fairbanks and Scott Abbott this translation differs from others in an attempt to be both more accurate than previous translations and also more accessible. GENETICS wishes to thank Scott Abbott and Daniel J. Fairbanks for their labors in presenting the scientific community with this new translation.

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These were largely done in support of the four to eight radiosondes launched each day as the ship travelled from Hawaii to TAO buoy locations along longitudes 140° W and 125° W and then back to port in San Diego, California. The rapid nature of these remote field deployments led to some specific challenges in addition to those common to many surface data collection efforts. This paper documents the two deployments as well as the steps taken to evaluate and process the data. The results are 10 two multi-week surface meteorology data products and one accompanying set of surface fluxes, all collected in the core of the east-central Pacific's extremely warm waters. These data sets, plus metadata, are archived at the NOAA's National Centers for Environmental Information (NCEI) and free for public access: surface meteorology from Kiritimati Island