The efficacy of an antimalarial drug depends on its ability to inhibit or interrupt essential life functions of the malaria parasite. The resurgence of malaria in various areas of the world, combined with the expansion of chloroquine resistant infections in the late 60s, focused attention on the development of in vitro tests which would permit the objective measurement of the sensitivity of human plasmodia to antimalarial drugs. After the achievement of continuous cultivation of Plasmodium falciparum in 1976 in appropriate media (Trager & Jensen 1976, Haynes et al. 1976), Rieckmann et al. (1978 adapted this methodology to produce a microculture procedure for the assessment of schizont maturation in a short-term test using chloroquine and mefloquine. Schizont maturation tests are essentially limited in their application to synchronous plasmodial infections or to those in which only one asexual growth form is generally found in the peripheral blood as the test studies the effect of drug on the maturation of parasite from ring to schizont stage. In human malarias this fact restricts their use to P. falciparum infection as only ring stage parasites are found in peripheral blood due to sequestration of erythrocytes containing advanced stages in capillaries. In this case, the schizont maturation test is widely applied and the microculture procedure has been the standard method employed in the global baseline assessment and monitoring of drug response (Lopez-Antunano & Wernsdorfer 1979, WHO 1984, Draper et al. 1985, Afari et al. 1993, Mberu et al. 2000. Since schizont maturation tests require uniform ring stage parasites, the asynchronous P. falciparum cultures are synchronized by chemical treatment. In this report we made an attempt to study the time course of in vitro maturation of sorbitol synchronized ring stage parasites of a culture adapted P. falciparum strain and compare these results with those obtained with in vitro maturation of a monkey malaria parasite, P. knowlesi, which has a unique quotidian periodicity and high natural synchronicity infection in monkeys (Collins 1988, Cogswell 2000. These features of the simian parasite add to the advantages of this species for wider application in chemotherapeutic studies.A culture adapted strain (FCD-3) of P. falciparum obtained from the International Center for Genetic Engineering and Biotechnology, New Delhi, India and W 1 strain of the simian parasite, P. knowlesi which was originally provided by Prof. PC Garnham (England) in 1976 and has been maintained at Central Drug Research Institute via blood induced passages in rhesus monkeys as well as cryopreservation in liquid nitrogen were used in the present study. Blood induced passages were carried out by intravenous inoculation of healthy rhesus monkeys 902 902 902 902 902Maturation of P. falciparum and P. knowlesi • SD Srinivas, SK Puri with 1 ml of infected rhesus blood in citrate anticoagulant solution (pH 7.4) from another infected monkey or 1 ml of revived parasites that were cryopreserverd (Rowe et al. 1968) ...

The present clinical study was attempted to evaluate biometrically the predictability of free rotated papilla autograft for multiple shallow gingival recessions. Materials and methods Fifteen systemically healthy patients with multiple gingival recessions underwent the procedure the probing depth, percentage of root coverage width of keratinized gingiva, width of attached gingiva were recorded at baseline 3 and 12 months. Results All parameters significantly improved from baseline to 12 months. The mean probing depth 1 mm ± 0 mm at baseline which was increased to 1.175 ± 0.245 mm at the end of 3 months and remained same at 12 months. The mean gingival recession was 2.35 ± 0.516 mm at baseline which was improved to 0.425 ± 0.245 mm at the end of 3 months and remained same at 12 months. The mean width of keratinized gingiva was 1.157 ± 0.245 mm at baseline which was improved to 3.15 ± 0.489 mm at the end of 3 months and remained same at 12 months. The mean width of attached gingiva 0.175 ± 0.245 mm at baseline which was improved to 1.975 ± 0.415 mm at the end of 3 months and remained same at 12 months. Conclusion The mucogingival surgery resulted in achieving high degree of success and predictability as well as an excellent esthetic outcome. Clinical significance Free rotated papilla autograft is a predictabe treatment modality for multiple shallow gingival recessions. How to cite this article Koganti VP, Chandrashekhar L, Srinivas SD, Kumar MK. The Free Rotated Papilla Autograft— A Bilaminar Procedure for the Coverage of Multiple Shallow Gingival Recessions: A Biometric Evaluation. J Contemp Dent Pract 2011;12(4):245-251.