No abstract

Solvothermal reactions of manganese(II) chloride tetrahydrate with a bis-tetrazole ligand, 2,6-di(1H-tetrazol-5-yl)naphthalene (H(2)NDT), in N,N'-dimethylformamide (DMF)/MeOH mixed solvent at two slightly different temperatures, 75 and 100 °C, led to two different metal-organic frameworks (MOFs), [Mn(II)(3)O(HNDT)(2)(NDT)(DMF)(3)] (1) and [Mn(II)(5)O(2)(HNDT)(2)(NDT)(2)(DMF)(8)] (2), with different net topologies. Single-crystal X-ray diffraction studies reveal that 1 is constructed from an unprecedented trinuclear building block, [Mn(II)(3)O(CN(4))(6)], as a 6-connected trigonal prismatic secondary building unit (SBU) of topological D(3h) site symmetry, and that the ligand in the HNDT(-1)/NDT(2-) deprotonation states is a linker, where two tetrazole (CN(4)) groups of the ligand are connected via a rigid naphthyl group. The tetrazole groups in 1 adopt a 1,2-μ-bridging mode with the manganese(II) ions to form a μ(3)-oxo trinuclear SBU. The trigonalprismatic SBU in 1 is connected to six neighboring SBUs to form a three-dimensional MOF of acs net topology. 2 is constructed from an unprecedented pentanuclear building block, [Mn(II)(5)O(2)(CN(4))(8)], as an 8-connected tetragonal prismatic SBU of topological D(4h) site symmetry. The tetrazole groups in 2 adopt monodentate, 1,2-μ- and 2,3-μ-bridging bidentate and 1,2,3-μ-bridging tridentate binding modes with the manganese(II) centers to form a bis-μ(3)-oxo pentanuclear SBU of local C(2) site symmetry. The tetragonal prismatic SBU in 2 is connected to eight neighboring SBUs to form a 3-D MOF of bcu net topology.

The antioxidant activity was determined by TBA (thiobarbituric acid) method as described previously (Lin and Chang, 2000;Kim et al., 2003). Reaction mixture contained 0.6 ml of 20 mM SPB, 1 ml linoleic acid emulsion (0.1 ml linoleic acid; 99%, Sigma, 0.2 ml Tween 20 and 19.7 ml deionized water) and 0.2 ml of sample. Lipid peroxidation was started by addition of FeSO 4 (0.2 ml, 0.01%) and H 2 O 2 (0.2 ml, 0.56 mM), and incubated in water bath for 6 h at 37°C. Trichloroacetic acid (TCA; 0.2 ml, 4%), TBA (2 ml, 0.8%) and butylated hydroxytoluene (BHT; 0.2 ml, 0.4%) were then added, and the mixture was boiled at 100°C for 20 min. The antioxidant activity was

Continuous positive airway pressure treatment aggravates airway obstruction in patients with epiglottis collapse. In these patients, partial epiglottectomy can resolve epiglottis collapse by partial excision of the obstructed epiglottis. However, patients with epiglottic collapse usually have simultaneous obstructions on multiple levels, such as the soft palate, base of the tongue, etc. Therefore, sleep apnea cannot be controlled merely by resolving epiglottis collapse. The use of additional continuous positive airway pressure treatment after partial epiglottectomy is considered essential. However, no studies have yet evaluated the effect of partial epiglottectomy on continuous positive airway pressure treatment. In this study, we report on 2 patients with obstructive sleep apnea who underwent partial epiglottectomy. These 2 patients used autotitrating positive airway pressure treatment pre-and postoperatively. The present case report will provide insight into the effects of partial epiglottectomy on the use of positive-pressure devices.

We selected a Lactobacillus spp. from Korean healthy infant feces based upon their antioxidant activity. This strain was identified as Lactobacillus gasseri by 16S rDNA sequencing, and named Lactobacillus gasseri NLRI-312. In the present study, we investigate the protective effect of this strain on the H 2 O 2 induced damage to cellular membrane lipid and DNA in Jurkat cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA (malondialdehyde) was measured, and DNA damage was tested by the comet assay. We also examined probiotic properties including tolerance to acid and bile, antibiotic resistance. From the results obtained, the supplementation of Jurkat cells with NLRI-312 decreased in DNA damage, while no effect was shown on MDA decrease. In probiotic properties, this strain was resistance to both acid and bile, showed considerably higher survival when incubated in pH 2 or 1% bile salts (w/v). We concluded that the NLRI-312 could be used as potential probiotic bacteria, with the effect of reducing DNA damage induced by H 2 O 2 .