Higher cyclorrhaphan flies including Drosophila develop a single extraembryonic epithelium (amnioserosa), which closes the germband dorsally. In most other insects two extraembryonic epithelia, serosa and amnion, line the inner eggshell and the ventral germband, respectively. How the two extraembryonic epithelia evolved into one is unclear. Recent studies have shown that, in the flour beetle Tribolium and in the milkweed bug Oncopeltus, the homeobox gene zerknü llt (zen) controls the fusion of the amnion with the serosa before dorsal closure. To understand the origin of the amnioserosa in evolution, we examined the expression and function of zen in the extraembryonic tissue of lower Cyclorrhapha. We show that Megaselia abdita (Phoridae) and Episyrphus balteatus (Syrphidae) develop a serosa and a dorsal amnion, suggesting that a dorsal amnion preceded the origin of the amnioserosa in evolution. Using Krü ppel (Kr) and pannier (pnr) homologues of Megaselia as markers for serosal and amniotic tissue, respectively, we show that after zen RNAi all extraembryonic tissue becomes indistinguishable from amniotic cells, like in Tribolium but unlike in Drosophila, in which zen controls all aspects of extraembryonic development. Compared with Megaselia and Episyrphus, zen expression in Drosophila is extended to cells that form the amnion in lower Cyclorrhapha and is down-regulated at the developmental stage, when serosa cells in lower Cyclorrhapha begin to expand. These expression differences between species with distinct extraembryonic tissue organizations and the conserved requirement of zen for serosa development suggest that the origin of an amnioserosa-like epithelium was accompanied by expression changes of zen.Megaselia ͉ Episyrphus ͉ Drosophila ͉ EvoDevo ͉ homology

This paper analyzes the packet loss and delay performance of an arrayed-waveguide-grating-based (AWG) optical packet switch developed within the EPSRC-funded project WASPNET (wavelength switched packet network). Two node designs are proposed based on feedback and feed-forward strategies, using sharing among multiple wavelengths to assist in contention resolution. The feedback configuration allows packet priority routing at the expense of using a larger AWG. An analytical framework has been established to compute the packet loss probability and delay under Bernoulli traffic, justified by simulation. A packet loss probability of less than 10 9 was obtained with a buffer depth per wavelength of 10 for a switch size of 16 inputs-outputs, four wavelengths per input at a uniform Bernoulli traffic load of 0.8 per wavelength. The mean delay is less than 0.5 timeslots at the same buffer depth per wavelength.

No abstract

A numerical study was conducted to characterize the probability and intensity of storm surge hazards in Canada’s western Arctic. The utility of the European Centre for Medium-Range Weather Forecasts Reanalysis 5th Generation (ERA5) dataset to force numerical simulations of storm surges was explored. Fifty historical storm surge events that were captured on a tide gauge near Tuktoyaktuk, Northwest Territories, were simulated using a two-dimensional (depth-averaged) hydrodynamic model accounting for the influence of sea ice on air-sea momentum transfer. The extent of sea ice and the duration of the ice season has been reducing in the Arctic region, which may contribute to increasing risk from storm surge-driven hazards. Comparisons between winter storm events under present-day ice concentrations and future open-water scenarios revealed that the decline in ice cover has potential to result in storm surges that are up to three times higher. The numerical model was also used to hindcast a significant surge event that was not recorded by the tide gauge, but for which driftwood lines along the coast provided insights to the high-water marks. Compared to measurements at proximate meteorological stations, the ERA5 reanalysis dataset provided reasonable estimates of atmospheric pressure but did not accurately capture peak wind speeds during storm surge events. By adjusting the wind drag coefficients to compensate, reasonably accurate predictions of storm surges were attained for most of the simulated events. The extreme value probability distributions (i.e., return periods and values) of the storm surges were significantly altered when events absent from the tide gauge record were included in the frequency analysis, demonstrating the value of non-conventional data sources, such as driftwood line surveys, in supporting coastal hazard assessments in remote regions.