Supplementary data are available at Bioinformatics online.

SignificanceDespite the fundamental importance of the surfaceome as a signaling gateway to the cellular microenvironment, it remains difficult to determine which proteoforms reside in the plasma membrane and how they interact to enable context-dependent signaling functions. We applied a machine-learning approach utilizing domain-specific features to develop the accurate surfaceome predictor SURFY and used it to define the human in silico surfaceome of 2,886 proteins. The in silico surfaceome is a public resource which can be used to filter multiomics data to uncover cellular phenotypes and surfaceome markers. By our domain-specific feature machine-learning approach, we show indirectly that the environment (extracellular, cytoplasm, or vesicle) is reflected in the biochemical properties of protein domains reaching into that environment.

Context. Debris discs are a consequence of the planet formation process and constitute the fingerprints of planetesimal systems. Their solar system counterparts are the asteroid and Edgeworth-Kuiper belts. Aims. The DUNES survey aims at detecting extra-solar analogues to the Edgeworth-Kuiper belt around solar-type stars, putting in this way the solar system into context. The survey allows us to address some questions related to the prevalence and properties of planetesimal systems. Methods. We used Herschel/PACS to observe a sample of nearby FGK stars. Data at 100 and 160 μm were obtained, complemented in some cases with observations at 70 μm, and at 250, 350 and 500 μm using SPIRE. The observing strategy was to integrate as deep as possible at 100 μm to detect the stellar photosphere. Results. Debris discs have been detected at a fractional luminosity level down to several times that of the Edgeworth-Kuiper belt. The incidence rate of discs around the DUNES stars is increased from a rate of ∼12.1% ± 5% before Herschel to ∼20.2% ± 2%. A significant fraction (∼52%) of the discs are resolved, which represents an enormous step ahead from the previously known resolved discs. Some stars are associated with faint far-IR excesses attributed to a new class of cold discs. Although it cannot be excluded that these excesses are produced by coincidental alignment of background galaxies, statistical arguments suggest that at least some of them are true debris discs. Some discs display peculiar SEDs with spectral indexes in the 70-160 μm range steeper than the Rayleigh-Jeans one. An analysis of the debris disc parameters suggests that a decrease might exist of the mean black body radius from the F-type to the K-type stars. In addition, a weak trend is suggested for a correlation of disc sizes and an anticorrelation of disc temperatures with the stellar age.

The origin of hot subdwarf B stars (sdBs) is still unclear. About half of the known sdBs are in close binary systems for which common envelope ejection is the most likely formation channel. Little is known about this dynamic phase of binary evolution. Since most of the known sdB systems are single-lined spectroscopic binaries, it is difficult to derive masses and unravel the companions' nature, which is the aim of this paper. Due to the tidal influence of the companion in close binary systems, the rotation of the primary becomes synchronised to its orbital motion. In this case it is possible to constrain the mass of the companion, if the primary mass, its projected rotational velocity as well as its surface gravity are known. For the first time we measured the projected rotational velocities of a large sdB binary sample from high resolution spectra. We analysed a sample of 51 sdB stars in close binaries, 40 of which have known orbital parameters comprising half of all such systems known today. Synchronisation in sdB binaries is discussed both from the theoretical and the observational point of view. The masses and the nature of the unseen companions could be constrained in 31 cases. We found orbital synchronisation most likely to be established in binaries with orbital periods shorter than 1.2 d. Only in five cases it was impossible to decide whether the sdB's companion is a white dwarf or an M dwarf. The companions to seven sdBs could be clearly identified as late M stars. One binary may have a brown dwarf companion. The unseen companions of nine sdBs are white dwarfs with typical masses. The mass of one white dwarf companion is very low. In eight cases (including the well known system KPD1930+2752) the companion mass exceeds 0.9 M , four of which even exceed the Chandrasekhar limit indicating that they may be neutron stars. Even stellar mass black holes are possible for the most massive companions. The distribution of the inclinations of the systems with low mass companions appears to be consistent with expectations, whereas a lack of high inclinations becomes obvious for the massive systems. We show that the formation of such systems can be explained with common envelope evolution and present an appropriate formation channel including two phases of unstable mass transfer and one supernova explosion. The sample also contains a candidate post-RGB star, which rotates fast despite its long orbital period. The post-RGB stars are expected to spin-up caused by their ongoing contraction. The age of the sdB is another important factor. If the EHB star is too young, the synchronisation process might not be finished yet. Estimating the ages of the target stars from their positions on the EHB band, we found PG 2345+318, which is known not to be synchronised, to lie near the zero-age extreme horizontal branch as are the massive candidates PG 1232−136, PG 1432+159 and PG 1101+249. These star may possibly be too young to have reached synchronisation. The derived large fraction of putative massive sdB binary systems ...