A straightforward epitope imprinting procedure is used to prepare IgG affinity membranes directly applicable to mAb capture under near process realistic conditions.

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In this work we introduce novel synthetic methods for modification of macroporous polypropylene (PP) membrane with poly(2-aminoethyl methacrylate) (polyAEMA) and subsequent anchoring Schiff base with the aim of adsorbing specific metal ions from aqueous solution. The Schiff base synthesis on the surface of PP membrane was done by a sequence of reactions. First the hydrophobic character of commercial PP membrane (pore diameter 0.4 µm) had been modified via UV irradiation-induced "grafting-from" of poly(2-hydroxyethyl methacrylate) (polyHEMA). The hydroxyl groups of poly(HEMA) were then reacted with the pre-synthesized photoinitiator 4-ethoxy-5-oxo-4,5-diphenylpentanoyl bromide. UV-irradiation was thereafter used for "grafting-from" of poly(2-aminoethyl methacrylate) (polyAEMA). The free amino groups this grafted comb-like brush layer on the surface were reacted with salicylaldehyde to form Schiff base. ATR-FTIR spectroscopy was carried out to determine the functional groups introduction and conversion. Scanning electron microscopy (SEM) images showed the changes between unmodified and modified membrane. Specific surface area was determined by nitrogen adsorption and BET analysis, and water permeability was also measured.The efficiency of membrane adsorbers with Schiff base in the grafted layer in binding Cu (II) ions was determined by atomic absorption spectroscopy. Overall, the established