This in vitro study aims to evaluate three different base materials (acetal, heat-polymerized, and auto-polymerized resins) on L-929 mouse fibroblast cells over 1 h-, 1-, 3-, 5-, 7-day periods. The hypothesis was that acetal resin would show higher cytotoxic effect than heat-polymerized and auto-polymerized acrylic resins, as it seems possible that residual formaldehyde might be leaching from the material into the cell culture medium. The samples were produced according to the manufacturer's protocol. Then they were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 1 h, 1, 3, 5, 7 days. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L-929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control. Statistical significance was determined by one-way ANOVA. Tukey and Tamhane tests were used as a post-hoc method to determine differences among the groups. Statistically significant difference was found among test groups at all time incubation periods (p = 0.000). The auto-polymerized resin performed higher cytotoxic effect than heat-polymerized resin and it was statistically significant at 1-day period (p < 0.05). The highest cytotoxic effect of acetal resin was observed at 5-day incubation period. In conclusion, the hypothesis was verified, since acetal resin showed more cytotoxic effect on the 3rd, 5th, and 7th days than heat- and auto-polymerized resins. Cell survival rates (% of control) of acetal resin were 58, 54, and 60%, respectively.

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Purpose The purpose of this study is to test the shear-bond-strengths of auto-mixed and manual-mixed self-adhesive resin cement to dentin on long-term high-altitude pressure. Design/methodology/approach Human molars were embedded in acrylic resin. Sixty composite resin discs were obtained. The composite resin discs were bonded to dentin using hand-mixed and auto-mixed self-adhesive resin cement. After cementation, the samples were stored in artificial saliva and divided into two subgroups (n = 30), hypobaric pressure and the atmospheric pressure group. The specimen underwent three pressure cycles per day for 100 days. The failure types were evaluated after debonding with scanning electron microscopy. The shear bond strength was tested with Universal Testing Machine. Analysis of variances/Tukey post hoc tests were used for statistical analysis. Groups were also evaluated by the Weibull modulus. Findings Regardless of hypobaric pressure changes, the highest bond strength was examined in auto-mixed Panavia SA samples. A significant difference was found in both auto-mixed MaxCem EC and hand-mixed RelyX U200 group after exposure to hypobaric pressure compared to the control group. Originality/value The luting cement-type, mixing methods of cements and environmental pressure changes significantly influence the bond strengths. Dentists can use auto-mixed self-adhesive resins in patients likely to be exposed to hypobaric pressure.

Introduction: The purpose of this study was to investigate the dentin bond strength of composite resins in response to environmental pressure changes. Methods: Ninety extracted human molar teeth were used. A mould (3 mm x 4 mm) was adapted on dentin, resin composites (conventional [n = 30] and single-shade composites [Ohmnicroma] [n = 30]) were filled in two increments of 2 mm. The bulk-fill composites (n = 30) were filled with one 4 mm increment. The specimens were stored for 30 days in artificial saliva. The specimens were exposed to hyperbaric pressure (283.6 kPa; 2.8 atmospheres absolute [atm abs]) or hypobaric pressure (34.4 kPa; 0.34 atm abs) once daily for 30 days and the control group was stored at atmospheric pressure for 30 days. The bond strength was tested with a universal testing machine and the failures were examined with a stereomicroscope and scanning electron microscope. Statistical analyses were performed using analysis of variance with post hoc tests, and the Weibull analysis. Results: Regardless of environmental pressure changes, the bulk-fill composites showed the highest bond strength. There was no significant difference in bond strength between the hypobaric and atmospheric pressure (control) groups after 30 days in all resins. The hyperbaric group showed lower bond strength for bulk-fill composites than the control group. Conclusions: Dentists experienced in diving and aviation medicine should definitely take part in the initial and periodic medical examinations of divers and aircrew to give appropriate treatment. Bulk-fill composite resins can be preferred in divers and aircrew due to high bond strength values.

Purpose The purpose of this study was to investigate the effect of the environmental pressure changes on the bond strength between zirconia ceramics and adhesive resin cement. Design/methodology/approach In total, 40 rectangular-shaped zirconium-oxide ceramic specimens were prepared. For surface modification, all zirconia specimens were sandblasted with 50 μm alumina particles. The composite resin discs were bonded to modified zirconia surfaces with resin cement. The specimens were divided into four groups; hyperbaric, hypobaric, hyperbaric + hypobaric and control group. The specimen underwent pressure cycles for 30 days. The shear bond strength test was performed by using the universal testing machine, and failures of the debonded samples were examined with scanning electron microscopy and light microscope. Findings No significant difference in bond strength was found between the hyperbaric, hypobaric and control groups after 30 days (p > 0.05). However, there was a significant difference in the hyperbaric + hypobaric group compared to the control group (p = 0.022). Also, the Weibull modulus was highest in control group and lowest in the hyperbaric + hypobaric group. Originality/value Barometric changes due to flying followed by diving may have an adverse effect on the retention of zirconia ceramics. Care should be taken in the selection of materials for dental treatment of people who are exposed to environmental pressure changes.