The comparative study of the effects of 5.8 mW/cm(2) Millimeter Waves (MMW) and near Infrared (IR) irradiation on thermal properties, specific adsorption rate (SAR), specific electrical conductivity (SEC) and hydrogen peroxide (H(2)O(2)) content of distilled water (DW), and physiological solutions (PS) was performed. The thermal effect of MMW irradiation appeared only after the first minute of irradiation, while the IR heating started from the first minute of irradiation. The heat fusion of frozen MMW-treated DW and PS was significantly less than sham and IR-treated DW and PS. MMW irradiation had time-dependent elevation effect on water SEC and SAR, which was accompanied by the increase of H(2)O(2) formation in it. We suggest that the MMW-induced vibration of water dipole molecules caused the non thermal changes of physicochemical properties of DW and PS, which promote the formation of H(2)O(2) in water.

The effects of dark -(Ev=0 lux) and low-background radiation (BGR), where R<1μRongen/h, on physicochemical properties (specific electrical conductivity, heat fusion, hydrogen peroxide (H2O2), and oxygen contents) of distilled water (DW) and physiological solution (PS) at 4°C and 18°C were studied. The incubation of DW and PS samples in dark and in low BGR (under dark) medium at 4°C and 18°C brings to changes of their physicochemical properties compared with DW and PS samples incubated in light and normal BGR condition (Ev=500-550 lux and R=17 μRoentgen/h). The observed changes of DW and PS properties depended on their initial temperature, density and ionic composition. It is suggested that water molecules dissociation and ions hydration are sensitive to illumination and BGR. Therefore, the cell-bathing medium can be considered as a messenger through which direct and non direct (by modulating of others factors-induced effects) influences of illumination and BGR on cell metabolism are realized.

At present, when the level of background ionizing radiation is increasing in a number of world locations, the problem of the study of biological effect of high background radiation becomes one of the extremely important global problems in modern life sciences. The modern research in biophysics proved that water is a most essential target, through which the biological effects of ionizing and non-ionizing radiations are realized. Therefore, there is no doubt about the strong dependency of non-ionizing radiation-induced effect on the level of background radiation. Findings have shown that illumination and background radiation have a strong modulation effect on infrasound-induced impacts on water physicochemical properties, which could also have appropriate effect on living organisms.

The effects of extremely low-frequency electromagnetic field (ELF EMF) and ELF-modulated millimeter waves (MMW) on physicochemical properties of physiological solution (PS) at different environmental medium were studied. The existence of frequency ''windows'' at 4 Hz and 8 Hz frequencies of ELF EMF and ELF-modulated MMW which have different effects on heat fusion period (HFP), hydrogen peroxide (H 2 O 2 ) formation and oxygen (O 2 ) content of water solution and different dependency on temperature, background radiation (BGR) and illumination was shown. The obtained data allow us to suggest that cell bathing medium serving as a target through which the biological effects of ELF EMF and ELF-modulated MMW on cells are realized is extra sensitive to environmental factors. Therefore, the variability of experimental data on EMF biological effects, obtained in different laboratories can be explained by different environmental conditions of experiments, which very often are not considered adequately.