Background: Several researches have shown that therapeutic compounds or phytochemicals from natural sources are important in the food as it is valuable in pharmaceutical industries, due to their fewer side effects and potent against various diseases. Curcumin, a major polyphenol derived from turmeric spice, which used in many foods, has a wide range of biological activities, with quite safety. Objective: The goal of this study was to investigate the antioxidant, urate-lowering, and anti-inflammatory effects of pure curcumin. Methods: antioxidant effect (radical-scavenging and reducing abilities assays) and for preventive antioxidant effect with metal chelating assay, the urate-lowering was assayed on aspectrophotometer by measuring the inhibition of uric acid production by xanthine oxidase (XO) enzyme, and the anti-inflammatory effect was estimated using in vitro albumin denaturation inhibition. Results: Curcumin showed asignificant and good chain-breaking antioxidant effect, both in free radicalscavenging assays (Galvinoxyl radical, ABTS, and hydroxyl radical), and in reducing abilities methods (reducing power, Cupric ion reducing antioxidant capacity and O-phenanthroline assays). In preventive antioxidant effect, assessed with themetal chelating assay, curcumin showed significant effect but with high concentration compared with standard. In the xanthine/xanthine oxidase system, curcumin significantly inhibited uric acid production (IC50= 0.71 ± 0.06 mg/mL). Regarding anti-inflammatory activity, curcumin showed significant inhibition of albumin denaturation with an IC50 value of 1181.69 ± 1.11µg/mL. Conclusion: These results indicated that curcumin showed promising antioxidant, anti-gout and antiinflammatory properties and might be used as potential, natural drugs against oxidative and inflammationrelated diseases.

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Aim: This study aims the investigation of the potential effect of pure curcumin on the inhibition of different enzymes which are involved in several diseases. Background: Several chronic diseases such as Alzheimer’s, diabetes, and Parkinson’s disease are related to oxidative stress and enzyme activity. Today, various plant origin products are stated as beneficial against several chronic diseases with secondary metabolites such as phenolic compounds. Curcumin, a polyphenol yellow-orange pigment present in turmeric spices, has a wide range of biological activities, with quite a safety. Objective: This study was aimed to investigate the antioxidant and inhibitory potential against key enzymes involved in human pathology, namely Alzheimer's disease (Acetylcholinesterase (AChE), and Butyrylcholinesterase (BChE)), diabetes (α-glucosidase) and hyperpigmentation and Parkinson’s diseases (Tyrosinase) of curcumin. Methods: 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH●) and hydrogen peroxide radicals (H2O2) assays were used to evaluate the antioxidant capacity of curcumin, enzyme inhibitory activity was evaluated using in vitro standard procedures. Results: Curcumin exhibited an excellent antioxidant effect with an IC50 value significantly less than the reference, Vit C. In enzyme inhibitory activity, curcumin demonstrated excellent inhibitory activity against AChE, BChE and α-glucosidase. The finding showed that curcumin was significantly less than the reference galantamine against AChE, but was significantly more than the references galantamine and acarbose against BChE and α-glucosidase, respectively. Whereas for anti-tyrosinase activity, curcumin displayed weak inhibitory activity compared with the standard inhibitor, Kojic acid.