SUMMARYThe larvae-to-adult development on the life cycle of zoonotic parasitic tapewormTaenia soliuminvolves striking -but clinically unappreciated-events with pivotal importance in cestode biology. Unlike the ones related to the intermediate host, the early-adult stages can be addressedin vitrooffering a useful model to study evagination, strobilation and worm development. In the absence of a stage-specific transcriptome, postgenomic data exploration followed by single-gene relative expression analysis by RT-qPCR (reverse transcription-quantitative PCR) are useful strategies to gather information on the regulation of genes of interest during parasite development. However, this approach requires the validation of an endogenous reference gene (RG) to achieve accurate comparisons.Therefore, we analyzed the expression stability of 17 candidate RGs on the context of the early-adult stages ofT. soliumclassified as non-evaginated and evaginated larvae (cysts). The comprehensive tool RefFinder ranked malate dehydrogenase as the most stable gene within these conditions, and its suitability for relative quantification was validated by normalizing the expression of the transporter TGTP1 gene, known for being upregulated upon evagination. This study is the first attempt in finding reliable normalization standards for transcript exploration in genus Taenia.