A flexible, patch-type strain sensor is described for continuous monitoring of pulse waves. The proposed sensor exploits the piezo-resistivity of the conductive polymer, polyaniline (PANI), to detect dynamic volume changes in blood vessels owing to pulse waves. The proposed PANI film was fabricated through electrodeposition, which is considered as a suitable low-cost technique for mass production in the sensor manufacturing industry. Thus, it is prospective for a disposable wearable sensing system solution in remote healthcare applications. Besides, a flexible sensor packaging can be achieved by laminating the PANI films and an ECOFLEX elastomer to the film bandage. The proposed PANI sensor has high sensitivity (gauge factor of 74.28) and linearity (R 2 = 0.99). It also showed a high correlation with commercially available photoplethysmography (PPG) sensor with the small bias and confidence interval to the PPG sensor: bias < 0.1% and confidence interval < 3% for all subjects. Moreover, the proposed PANI sensor was tested for prospective circulatory system-related applications such as measuring heart rate, stiffness index, and pulse transit time. Finally, the proposed study suggests that the proposed PANI sensor is a promising candidate for continuous, long-term, unobtrusive pulse wave monitoring, which can provide real-time insights into an individual's health status.

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Heterotypic interactions between cells are crucial in various biological phenomena. Particularly, stimuli that regulate embryonic stem cell (ESC) fate are often provided from neighboring cells. However, except for feeder cultures, no practical methods are identified that can provide ESCs with contact-dependent cell stimuli. To induce contact-dependent cell stimuli in the absence of living cells, a novel method that utilizes cell-engineered nanovesicles (CNVs) that are made by extruding living cells through microporous membranes is described. Protein compositions of CNVs are similar to their originating cells, as well as freely diffusible and precisely scalable. Treatment of CNVs produced from three different stromal cells successfully induces the same effect as feeder cultures. The results suggest that the effects of CNVs are mainly mediated by membrane-associated components. The use of CNVs might constitute a novel and efficient tool for ESC research.