In yeasts, chitin is a minor componentof the cell wall, but at the budding cell stage, it is a main constituent of the primary septum between the mother and daughter cells.1} Among the chitin metabolite enzymes, chitin synthase is essential for the septum formation and its loss results in arrest of growth.2) On the other hand, the activity of chitinase also increases
Self-incompatibility in the cruciferous plant, Brassica, is strongly associated with glycoproteins, which are specific to S-locus and then termed S-glycoproteins; but their structures and functions are not yet known.1}In the preceding paper,2) we described the isolation, amino acid composition and N-terminal amino acid sequences of *S8, S9 and S12-glycoproteins from three different strains of B. campestris, and also reported that S8- appearing before 7 min were attributed to byproducts from reagents, and the peaks around 7.5min were mixtures of several minor saccharides and probably contained some byproducts of the major peaks.4) Therefore it was concluded that the carbohydrate chains of S8, S9 and S12-glycoproteins were commonly composedof two main substances, oligosaccharides A (la) and B (2a), which gave the
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